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腹膜透析流出液中人腹膜间皮细胞的培养 被引量：1

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摘要 目的建立从腹膜透析(PD)流出液中培养人腹膜间皮细胞(HPMC)方法。方法从10例PD患者PD流出液标本中收集HPMC并在体外进行原代培养,采用相差倒置显微镜、扫描电镜和免疫组化染色对培养的细胞进行鉴定。结果8例PD患者流出液中HPMC在体外原代培养成活,经相差倒置显微镜观察原代培养的HPMC呈典型的铺路石样外观;免疫组化显示培养的HPMC胞浆角蛋白、波形蛋白表达均阳性,白细胞CD45抗原阴性;扫描电镜见培养细胞表面有许多长短不一呈丝状的微绒毛。结论成功建立了从PD流出液中HPMC原代培养方法,该方法将对进一步PD的研究提供实验基础。
作者 戴宏 刘先英 张伯科 吴永贵 徐星铭 郝丽 钱浩 阚春明
机构地区 安徽医科大学第一附属医院肾脏内科 青岛市海慈医疗集团肾脏内科
出处 《安徽医科大学学报》 CAS 北大核心 2008年第4期464-466,共3页 Acta Universitatis Medicinalis Anhui
基金 安徽省教育厅自然科学研究项目(编号:2004kj200)
关键词 腹膜透析 细胞 培养的 腹膜/细胞学
分类号 R459.51 [医药卫生—治疗学] R321.2 [医药卫生—人体解剖和组织胚胎学]
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二级参考文献19

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共引文献10

同被引文献13

  • 1刘洪斌,李东华,郭世铎.腹腔粘连形成机制及治疗研究进展[J].中国中西医结合外科杂志,2005,11(1):84-86. 被引量：20
  • 2程永波,房殿春,郭立萍,王自强,罗元辉,周建嫦.一种简便的人正常胃黏膜上皮细胞原代培养方法[J].胃肠病学,2007,12(1):31-35. 被引量：4
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引证文献1

安徽医科大学学报
2008年 第4期

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