摘要
目的:观察雌激素对偏头痛大鼠头痛发作时行为学及中脑导水管周围灰质(PAG)区5-羟色胺(5-HT)表达的影响,探讨雌激素在偏头痛发病中的作用。方法:健康雌性Wistar大鼠24只,去卵巢后随机分4组:对照组(A组)、偏头痛组(B组)、低雌激素替代治疗组(C组)和高雌激素替代治疗组(D组)。1周后B、C、D组给予硝酸甘油10mg·kg-1皮下注射制备偏头痛模型,A组以同剂量花生油皮下注射,观察注射后大鼠行为学改变,注射2h后处死大鼠,5-HT免疫组化染色。结果:行为学表现,D组爪红、耳红、尾红程度较B组明显减轻,爬笼、搔头次数明显减少,C组与B组大鼠相比无明显差别;免疫组化染色,B组、C组、D组与A组比较,中脑导水管周围灰质5-HT阳性神经元增多(P<0.01),以腹外侧和背外侧明显,B组增加程度最明显,C组次之,D组增加程度最低,各组之间差异有显著性(P<0.01)。结论:雌激素能减少PAG区5-HT阳性神经元的激活,其作用有剂量依赖性,高雌激素能减轻偏头痛大鼠行为学改变,作用机制可能与雌激素影响参与偏头痛发作的神经活性物质有关。
Objective To observe the effects of estrogen on behavior and 5-HT in periaqueductal gray （PAG） in migraine model rats. Methods Tewnty-four ovariectomized Wistar rats were divided randomly into four groups： control group （Group A）, migraine group （Group B）, low dose estradiol-treated ovariectomized group （Group C）, high dose etradiol-treated ovariectomized group （Group D）. After 1 week, the rats in Group B, C and D were injected with nitroglycerine 10 mg·kg-1 subcutaneously to make migraine rat models, the rats in Group A were given peanut oil alike, and the behavior changes were observed. 2 h after injection, the rats were killed and the midbrains were separated and then 5-HT immunohistochemical staining was performed. Results Behavior.. compared with Group B, the degrees of red-calws, red-ears and red-tail rats in Group D relieved obviously, the times of climbing hutch and scratching head were much fewer, while the rats in group C showed no significant difference; Immunohistochemical staining., compared with Group A, the 5-HT-positive neurons expression in PAG of Group B and C were more obviously （P〈0.01）, especially in the ventrolateral and dorsolateral of PAG, the degree of 5-HT-positive neurons increased in PAG of Group B as most, Group C as more, while group D as a little. There were significant differences between various groups （P〈0. 01）. Conclusion Estrogen can reduce the degree of 5-HT-positive neurons activating in PAG, and its role in a dose-dependent manner, and high dose estrogen can decrease the changes of behavior of migraine rats, the mechanism may be related with that estrogen can influence the neuroactive substances taking part in migraine.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2008年第1期131-134,F0003,共5页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅基金资助课题(200705238)
