摘要
首次使用甲基化敏感扩增多态性(MSAP)技术分析两种小麦材料近等基因系TcLr19及其感病亲本Thatcher的甲基化水平,同时比较了苗期接种叶锈菌生理品种THTT前后基因组DNA胞嘧啶甲基化模式。60对选扩引物对接种前后的小麦DNA进行全基因组筛选,共得到3 554个片段。其中998个片段是两种甲基化模式中的一种,小麦近等基因系TcLr19及其感病亲本Thatcher的甲基化水平约为28.1%。在所有的引物中,并没有直接分离得到接菌前后的甲基化模式的差异。结果初步表明,叶锈菌可能并没有诱导植物基因组DNA胞嘧啶位点的甲基化模式变化。
DNA methylation is known to play an important role in the regulation of gene expression in eukaryotes. The wheat near-isogenic line TcLr19 and Thatcher is race-specific resistance and compatible in the interaction with Paccinia triticina respectively. Using methylation-sensitive amplified polymorphism（MSAP） analysis, we compared the patterns of cytosine methylation in TcLr19 and Thatcher that had been inoculated with Puccinia triticina, subjected to left untreated. In all, 3 554 DNA fragments, each representing a recognition site cleaved by either or both of two isoschizomers, were amplified using 60 pairs of selective primers. A total of 998 sites were found to be one of the methylated.The results suggest that no differences in cytosine methylation exist between seedlings inoculated and those unchallenged.
出处
《华北农学报》
CSCD
北大核心
2008年第4期28-31,共4页
Acta Agriculturae Boreali-Sinica
基金
国家“973”专项(2005CCA01600)
国家“十一五”支撑计划(2006BAD08A05)
