摘要
利用不同辐照剂量的60Co-γ射线对3个品种的大豆种子进行辐照处理,剂量分别为0(CK),50,100,150,200,250和300 Gy 7个水平,剂量率为1.2 Gy/min。根据大豆M1的成株率和M2农艺性状的变异率确定辐照的最佳剂量以及对M2的致变效应。结果表明:辐照处理对M1的农艺性状有抑制作用,其性状平均数与辐照剂量呈显著负相关,关系式为y=0.000 1x2-0.381x+102.9(R2=0.962 7**);品种间最佳剂量有差异,平均为144.3 Gy;不同辐照剂量对M2植株的株高、主茎节数、荚长、单株荚数、单株粒数和百粒重等都有不同程度的影响,剂量越大,影响越大;由于基因的突变,在M2植株中可发现一些超亲类型。
Gene mutation ratio could be improved by irradiation. To study the rote of irradiation techniques in the relationship between irradiation dosage and generation after irradiation is important. 3 cuhivars of soybean were irradiated by 6 irradiation dosages of ^60Co-γ rays,50,100,150,200,250,300 Gy and 0 Gy（ck）, respectively, and the ratio of irradiation dosage was 1.2 Gy/m in this paper. Based on the living plant ratio in M1 and the mutation effect in M2 ,the best irradiation dosage and the irradiation dosage in M2 were evaluated in the test.The results showed that the mean value of the agronomic characters which were control growth was in negative correlation with the irradiation dosage in the M1 generation by ^60Co-γ rays.The equation follows as y = 0.0001x^2 -0.381x ＋ 102.9（R^2 = 0.962 7^＊＊ ）;The best irradiation dosage was different in cultivars and their mean value was 144.3 Gy;and the plant height, main node number, pod length, pod and grain number per plant and hundred-grain-weight were affected by different irradiation dosage in M2, and the more irradiation dosage, the greater effect in a certain extent;gene mutation enabled the plants to exceed their parents in M2 generation.
出处
《华北农学报》
CSCD
北大核心
2008年第4期89-93,共5页
Acta Agriculturae Boreali-Sinica
基金
北京市自然科学基金(5052006)
北京市教委资助项目(KM200810020008)
关键词
大豆
辐照
农艺性状
致变效应
Soybean
Irradiation
Agronomic characters
Mutate effect
