摘要
在目前我国海洋生物中石油烃检测方法(GB17378.6-1998)基础上,对方法中的皂化温度和时间、萃取剂和溶剂的选择以及萃取过程的优化进行了研究。将海洋生物样品于38℃烘箱中经NaOH皂化10 h,能确保样品皂化完全且不受室温限制;用石油醚(30～50℃)代替氟里昂萃取,其效果更佳且减少了对环境的污染;蒸干后的残留物用石油醚(60～90℃)溶解,去水后用荧光分光光度计进行测定。分析结果表明,本方法的检测限为0.26 mg/kg,比目前采用的国标方法高3倍多;相对标准偏差不超过7.7%,加标回收率结果在88.5%～108.9%之间,适合于水产品中石油烃的检测。
Based on the present method for determination of petroleum hydrocarbon in aquatic products （GB 17378.6-1998）, the condition of saponification, extractant and solvent selection, and optimization of the extraction process were studied. Samples of marine organisms were NaOH-saponified in oven at 38 ℃ for 10 h, which ensured complete saponification as not affected by room temperature. Petroleum aether （30-50 ℃） was used as the extractant instead of Freon, so that the method was more effective and less harmful to the environment. The dried residue was dissolved in petroleum aether （60 - 90 ℃） and was determined by fluorescence spectrophotometer after dehydration. The result showed that the detection limit （DL） of this method was 0. 26 mg/kg , which was over 3 times higher than that of GB 17378.6-1998; the RSD of this method was less than 7. 7% and recovery ratio was between 88.5%-109.0%. Consequently, the method is fit for determination of total petroleum hydrocarbon in aquatic products.
出处
《海洋水产研究》
CSCD
北大核心
2008年第4期106-111,共6页
Marine Fisheries Research
基金
国家科技基础条件平台项目(2004DEA70880)
国家863计划项目(2007AA09Z438)共同资助
关键词
水产品
石油烃
荧光分光光度法
测定
Aquatic products Petroleum hydrocarbon Determination Fluorescence spectrophotometry
