摘要
目的观察丙烯酰胺亚慢性染毒对大鼠神经行为功能及电生理变化的影响。方法30只雄性SD大鼠随机分为3组(对照组、低和高剂量组)每组10只,分别以生理氯化钠、丙烯酰胺20、40mg/kg剂量腹腔注射,每周3次,共10周,测量大鼠体重、热板仪、甩尾、后肢撑力及神经传导速度等指标。结果中毒后大鼠体重减轻,从第4周开始高剂量组与对照组比较有统计学意义(P<0.05),第6周高剂量组与低剂量组比较有统计学意义(P<0.01),热板仪实验异常(高剂量组时间延长57%,低剂量组时间延长44%),甩尾实验时间延长(高剂量组延长38%),后肢展开距离明显加宽(高剂量组增加102%,低剂量组增加61%),胫神经和腓肠神经传导速度降低(感觉神经传导速度降低32%,运动神经传导速度降低33%)。结论丙烯酰胺可引起大鼠的热觉传导异常,运动神经损伤,传导速度降低。
Objective To observe the changes of neurobehavior function and eleetrophysiology in rats exposed to aerylamide. Methods Male SD rats （220～250 g） were randomly divided into 3 groups （ 10 rats per group）. The rats in group 1 were served as the control （NS only） and the other 2 groups were given acrylamide （20 or40 mg/kg ip, 3 times/week） for 10 weeks. Special tests included observations of nervous sensory to pain, measurements of hindlimb landing foot splay and electrophysiological changes in rats. Results The weight of the rats treated with acrylamide were reduced （ P 〈 0. 05 ） after 3 weeks of exposure. Compared with the control group, the hind paw-lick latency of rats at high-dose group was significantly prolonged by 57% （P 〈 0. 01 ）, the tail-flick lantency at high dose group was prolonged by 38%, hindlimb landing foot splay at low-dose and high-dose group were increased by 102% and 61%, respectively （P 〈0. 05）. Between ACR and the control groups there were significant differences in tibial-nerve motor nerve and sural-nerve sense nerve conduction velocity （P 〈 0. 05）. Conclusion ACR can significantly affect the function of sensory and motor nerve, which provides evidence for the neurotoxicity of ACR.
出处
《中国职业医学》
CAS
北大核心
2008年第4期278-280,共3页
China Occupational Medicine
