摘要
目的:利用RP-HPLC法建立陕西留坝西洋参药材的化学指纹图谱。方法:采用低压梯度洗脱方法进行色谱分离。实验测定28批药材并记录指纹图谱,不同样品之间的相似度采用国家药典委员会开发的中药色谱指纹图谱相似度评价系统计算。结果:测定28批陕西留坝西洋参样品,标定共有峰14个,其指纹图谱相似度均在0.9以上,特征峰的相对含量分布差异导致色谱概貌存在一定差异。结论:该法简便、快速、精密度高、重现性好,为留坝西洋参药材的定性鉴别及内在质量评价提供了科学依据。
Objective ： To establish a fingerprint analysis method for quality control of Panax quinquefolium from Liuba County in Shanxi Province by RP-HPLC. Methods ： A gradient mobile system was applied in the experiment. The fingerprints of different samples were compared by similarity evaluation software. Results ： The similarities among different samlpes were high. 14 peaks were identified as the characteristic fingerprints of Panax quinquefolium. All samples contained the same characteristic peaks, but the content difference of each peak among the samples resulted in the difference of their chromatograms. Conclusions ： This method is precise with good repeatability and good baseline separation of all the chromatographic peaks, so it can be effectively used to assess the quality of Panax quinquefolium.
出处
《中药材》
CAS
CSCD
北大核心
2008年第8期1146-1149,共4页
Journal of Chinese Medicinal Materials
