摘要
为研究氮源的种类和浓度对三角褐指藻(Phaeodactylum tricornutum)生长及脂肪酸组成的影响,用含有不同浓度的NO3-和NH+4在叉培养基中对三角褐指藻进行培养,测定了其生长及脂肪酸的组成.结果表明,以NH4+为氮源时三角褐指藻的比生长速率大于硝酸钠;在NH4+为2.1 mmol/L时三角褐指藻达到最高比生长速率.三角褐指藻的主要脂肪酸是14∶0、16∶0、16∶1和20∶5n-3(二十碳五烯酸,EPA).以NO3-为氮源时,EPA和高度不饱和脂肪酸(PUFAs)含量随着NO3-浓度增加先上升后下降,在1.0 mmol/L时的EPA占总脂肪酸质量分数比达到最高(16.9%).当氯化铵浓度大于3.9 mmol/L时,EPA的含量占总脂肪酸质量分数比随浓度的增加较大,在8.0 mmol/L达到26.1%.
Effects of nitrogen sources on the growth of Phaeodactylum tricornutum and production of polyunsaturated fatty acids （PUFAs） were investigated by culturing in ca-medium with various concentrations of NO3^- and NH4^＋. The algae grow faster with NH4^＋ than NO3^-. The highest specific growth rate was attained at 2.1mmol/L of NH4^＋. The major fatty acids of this algae are 14 ： 0, 16 ： 0, 16 ： 1 and 20 ： 5n-3 （Eiscosapentaenoic acid, EPA）. The production of EPA and PUFAs with NO3^- as the nitrogen source increased initially and then decreased. A maximum EPA content of 16.9 % wt was obtained at 1.0 mmol/L of NO3^-. With NH4^＋concentration 〉3.9 mmol/L, the production of EPA increased more sharply and achieved 26. 1% wt at 8. 0 mmol/L of NH4^＋.
出处
《中南民族大学学报（自然科学版）》
CAS
2008年第2期32-35,共4页
Journal of South-Central University for Nationalities：Natural Science Edition
关键词
氮源
三角褐指藻
脂肪酸
高度不饱和脂肪酸
二十碳五稀酸
nitrogen source
Phaeodactytuum tricornutum
fatty acids
polyunsaturated fatty acids （PUFAs）
Eiscosapentaenoic acid （EPA）
