摘要
用不同浓度的茶多酚液对玉米小斑病菌(Bipolaris maydis)、香蕉炭疽病菌(Colletotrichum musae)和莲腐败病菌(Fusarium oxysporumf.sp.)进行抑菌测定。结果表明:茶多酚对三种植物病原真菌生长和分生孢子萌发都具有极显著的抑制作用(P<0.01);不同浓度的茶多酚液对同种植物病原真菌的抑制作用不同,随着茶多酚浓度的增大,其抑制力增强,其中10和5 mg/mL抑制力最强;茶多酚对三种不同的植物病原真菌的抑制程度也不同,其中对玉米小斑病菌的抑制效果最好,10和5 mg/mL茶多酚稀释液的分生孢子萌发抑制率达100%,且原生质外溢,细胞畸变。其作用机理是破坏了菌体的细胞膜结构和抑制了CAT、POD酶活,使其丧失细胞膜的屏障和酶系的保护功能。
The inhibitive effects of different tea-polyphenol （TP） concentrations on Bipolaris maydis, Colletotrichum musae and Fusarium oxysporum f. sp. were tested. The results showed that TP significantly inhibited hyphal growth and spore germination of the 3 species of plant pathogenic fungi （P 〈0.01 ）. The inhibitive rate against the fungi changed, as the concentration of TP changed; the higher the concentration was, the higher the inhibitive rate was. Among the series concentrations, the 10 and 5 mg/mL TP solution had the highest inhibitive rate against the 3 fungi. However, the inhibitive efficiency of TP against each of the 3 fungi was different. Of the fungi ,Bipolaris maydis was inhibited most efficiently by TP;the 10 and 5 mg/mL TP solution inhibited 100% spore germination of the fungus, and caused protoplasm overflow and cell distortion. The action mechanism was that it destroyed the structure of cell membrane and strongly inhibited the enzyme activity of CAT, POD made the cells lose the protection of cell membrane barrier and enzyme system.
出处
《天然产物研究与开发》
CAS
CSCD
2008年第4期690-694,共5页
Natural Product Research and Development
基金
国家自然科学基金[(2003)30260050]
关键词
荼多酚
植物病原真菌
抑制作用
机理
tea-polyphenol
inhibitive action
plant pathogenic fungi
mechanism
