摘要
[目的]建立高效液相色谱-质谱法，分析金花葵花中金丝桃苷。[方法]以乙腈-浓度0．1％甲酸为流动相，流速0．6ml／min，经反相色谱柱Lichrospher C18（250mm×4．6mm，5μm）分离后进行质谱分析，采用正负电喷雾离子源同时进行信号采集。[结果]经HPLC／MS定性鉴定，金花葵花中含有金丝桃苷，含量测定的线性范围为10．0～300．0μg／ml，平均回收率为101．27％。[结论]该方法简便、快速、灵敏度高，可用于分析金花葵花中金丝桃苷的分离测定。
[ Objective] The hyperoside in flower of Hibiscus manihot L. was measured by HPLC-Mass spectrometry. [ Method] By means of aeetonitrile- 0.1% formic acid as mobile phase with flowing rate at 0.6 ml/min, exploiting Lichrospher C 18 （250mm ×4.6mm,5μm）, the tandem mass spectral acquisition was done in positive and negative electropray ionization. [ Result] The flower studied contained hyperoside. The linear range of this method was from 10.0 to 300.0 μg/ml. The average recovery was 101.27%. [ Conclusion] The method could be applied to determine hyperoside in the flower of Hibiscus manihot L. because of its briefness, rapidity, good sensitivity and recovery.
出处
《安徽农业科学》
CAS
北大核心
2008年第23期10028-10029,共2页
Journal of Anhui Agricultural Sciences
基金
江西省宜春市社会科学研究规划资助项目(jxyc2006kna016)
