摘要
比较了测定奶牛全血硒的微分脉冲催化极谱法和２，３─二氨基萘荧光法。结果：两种方法测得的血硒含量差异不显著（Ｐ＞０．０５），测值均符合饲料添加水平；但极谱法的最低检出浓度为０．１ｎｇ／ｍｌ，而荧光法则为１ｎｇ／ｍｌ，极谱法测定的精密度和准确度优于荧光法，但其线性范围比荧光法窄。从而提示：微分脉冲催化极谱法测定生物样品硒含量较为准确、简便、经济、省时，且污染少。
This paper reported the comparison between Differential PulseCatalytic Polarography(DPCP)and 2,3-diaminonaphathalene molecularfluorimetry(DAMF)for the determinaton of selenium in dairy cattle blood,The results indicate:There was no signiflcant difference between twomethods in blood selenium of dairy cattle.The results were bothconformed with feedstuff additive patterns.The detection limit of DPCPin selenium was 0.1 ng/ml,DAMF was 1 ng/ml.Theprccision and rccovcry of DPCP were better than those of DAMF.but theliner range of DPCP was more arrow than that of DAMF.DPCP was accurate,simple and convenient,low-priced,save time and less pollution.
出处
《黑龙江畜牧兽医》
CAS
北大核心
1997年第12期9-11,共3页
Heilongjiang Animal Science And veterinary Medicine
关键词
乳牛
全血
硒
测定
Differential Pulse Catalytie Polarography,2,3-Diaminonaphathalcne Molecular Fluorimetry,Dairy Cattle Blood,Selenium
