摘要
采用PCR技术获得了唇(Hemibarbus labeo)和花(H.maculatus)线粒体DNA细胞色素氧化酶(COⅡ)基因部分序列,将所得的COⅡ基因序列与4种取自GenBank的属鱼类同一基因序列进行了分析,以探讨这一序列在种质鉴定、种群遗传结构和分子系统发生研究中的应用价值.测序结果表明,在实际分析的600bp序列中,序列A+T含量(56.9%～57.8%)高于G+C含量,物种间共有变异位点116个,其中简约信息位点46个;唇和花2个地理种群间变异位点均为11个,唇种群间碱基替换均为转换,花的转换/颠换为8/3.以COⅡ基因片段序列为标记,用似刺鳊鮈(Paracanthobrama guichenoti Bleeker)作外群,构建了属鱼类的系统发生树,其拓扑结构显示2个地理种群浙江花单倍体Ⅰ(H1)与江苏花首先聚为一支,然后与浙江花单倍体Ⅱ(H2)形成一个单系群.韩国唇和日本为姐妹群,然后与中国花(浙江和江苏)相聚为一支.长吻和朝鲜单独聚为一支,表明2者的亲缘关系较近.结果同时表明,韩国唇与江苏花、日本和浙江花(H1)的遗传距离均为0.0084,结合形态特征鉴定,推测韩国唇和日本是中国花(浙江和江苏)的同物异名.
Partial sequences of the mitochondrial cytochrome oxidase unit Ⅱ （CO Ⅱ ）gene of 2 species of Hemibarbus proliferated by Polymerase Chain Reaction（PCR）, and other four species of Hemibarbus from GenBank were analyzed to determine the utility of this regions in identifying phylogeographic and phylogenetic structure. Nucleotide sequences of 600 bp in length were obtained from CO Ⅱ gene for 20 Hemibarbus specimens＇ representing populations of H. labeo and H. maculates from Oujiang River, with A＋T contents from 56.9% to 57.8%, which were obviously higher than G＋C contents. Analysis of sequence variation at the CO Ⅱ gene across five species revealed 116 variable sites, in which 46 were parsim-informative sites. Analysis of sequence variation across two H. labeo and H. maculatus populations revealed 11 variable sites, in both series. The base substitution was transition in H. labeo and the ratio of transition （Si） and transversion （Sv） was 8/3 in H. maculatus. The phylogenetic trees of Hemibarbus were reconstructed by using CO Ⅱ gene sequences as marker, and with Paracanthobrama guichenoti Bleeker as outgroup. Tree topologies indicated that haplotype I of H. maculatus（Zhejiang） and H. maculates（Jiangsu） formed a monophyletic group with the hapiotype Ⅱ of H. maculatus（Zhejiang）. H. labeo from South Korea and H. barbus were sister group, and then formed a clade with H. maculatus from China. H. longirostris and H. mylodon were formed a single clade, which suggested that the relationship between them were closer. The genetic distances between H. labeo from South Korea, and H. maculatus（Jiangsu） H. barbus and haplotype Ⅰ of H. maculatus（Zhejiang） were 0. 0084 in both cases. Combined together morphotogic characteristics, it was infered that H. labeo from South Korea and H. barbus should be treated as synonym of H. maculates from China.
出处
《浙江大学学报（理学版）》
CAS
CSCD
北大核心
2008年第5期580-585,共6页
Journal of Zhejiang University（Science Edition）
基金
浙江省自然科学基金资助项目(No.Y307445)
浙江省科技厅新苗人才计划资助项目(No.2007R40G2260026)
浙江省教育厅资助项目(No.20070552)
浙江省丽水市科技局资助项目(No.2007kjh2005)
