摘要
目的观察肝组织纤维化及炎症坏死与大鼠肝组织结缔组织生长因子(CTGF)表达的关系,探讨CTGF在肝纤维化形成中的作用。方法采用尾静脉注射人血白蛋白法复制免疫性肝纤维化模型,应用免疫组织化学及RT-PCR方法观察肝组织中CTGF的表达及其与肝纤维化和炎症活动的关系。结果①肝组织CTGF阳性细胞主要分布于汇管区、纤维间隔及近纤维间隔的肝窦细胞中,肝细胞始终为阴性。②随着肝纤维化的进展,从S0到S4肝组织CTGF及其mRNA表达逐渐增高,与S0组比较及4组之间两两比较差异均有显著性(P<0.05)。③随着肝组织炎症坏死的加重,从G0到G4肝组织CTGF及其mRNA表达逐渐增高,与G0组比较,G1组与G2组之间比较差异有显著性(P<0.05),而G3与G2组之间及G4与G3组之间则差异无显著性(P>0.05)。结论CTGF在肝纤维化形成过程中起重要作用。
[Objective] To reveal the pathogenesy of connective tissue growth factor （CTGF） in liver fibrosis by observing the relationship of liver fibrosis and inflammation and CTGF in liver tissue of experimental rat. [Methods] The immune liver fibrosis model in rats were established by injected human albumin into vena caudalis.The expression of CTGF in liver tissues were detected by immunohistoehemistry and RT-PCR. [Results] ①The positive cells of immunohistochemistry of CTGF distribute at portal area, fabric interval, juncture with liver parenchyma and peri.＇sinusoidal cells. It is negative in parenchymal hepatic cells and normal group. ②With the development of fibrosis, the expressions of CTGF and it＇s mRNA in liver tissues increase gradually from SO to S4. Compared to SO, S1, S2, S3 and S4 are all significantly higher （P 〈0.05）. And there are significant differences between each of them （P 〈 0.05）. ③With the development of inflammation and cellular necrosis, the expressions of CTGF and it＇s mRNA in liver tissues increase gradually from GO to G4. Compared to GO, G1, G2, G3 and G4 are all significantly higher （P 〈 0.05）. The difference between G1 and G2 are notable （P 〈0.05）. But the differences between G2 and G3, G3 and G4 are unnotable （P 〉0.05）. [Conclusions] CTGF pays an important role of the devolpment of liver fibrosis.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2008年第16期2312-2314,2318,共4页
China Journal of Modern Medicine
