摘要
目的观察板党对阿尔茨海默病(Alzheimer disease,AD)模型大鼠海马、皮质氧自由基和神经元微管相关蛋白(tau蛋白)表达的影响。方法于大鼠侧脑室注射冈田酸(Okadaic acid,OA)造模,用抗氧化剂维生素C(V,C)腹腔注射作阳性对照,测定正常对照组、阳性对照组、模型组、板党高、中、低剂量组海马、皮质超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、过氧化脂质(MDA)和tau蛋白的含量。结果模型组皮质SOD(114.98±22.90)、GSH-PX(13.11±1.78)和海马SOD(159.94±14.67)、GPK-PK(12.25±1.47)低于正常对照组(P<0.05,P<0.001),皮质MDA(5.38±2.76)t、au蛋白(174.97±12.89)和海马MDA(4.81±1.54)t、au蛋白(148.61±10.77)高于正常对照组(P<0.001)。板党各组与阳性对照组比较,SOD、GSH-PX、MDA和tau蛋白表达,在高(750 mg)、中(500 mg)剂量组差异有统计学意义(P<0.05),低(250 mg)剂量组差异无统计学意义(P>0.05)。结论板党能增强脑组织SOD、GSH-PX活性、减少脑组织MDA蓄积、降低脑脊液tau蛋白水平。
Objective To observe the effect of codonopsis pilosula （Enshi） on expression of oxygen free-radical and tau protein in gyrus hippocampi and cerebral cortex of rats with Alzheimer disease induced by okadaic acid. Methods The rat model of Alzheimer disease induced by okadaic acid was employed. Positive control and test group were injected with Vit. C. Codonopsis pilosula （Enshi） groups and control group＇s supreoxide dismutase （SOD）, glutathione peroxidase（GSH- PX）, malondialdehyde（MDA） and tau protein in gyrus hippocampi and cerebral cortex were measured. Results In contrast with control groups, concentrations of SOD （114.98 ± 22.90）, GSH - PX（13.11 ± 1.78）and SOD（159.94 ± 14.67）, GSH - PX （ 12.25 ± 1.47） in cerebral cortex and gyrus hippocampi of rats with Alzheimer disease were lower（ P 〈 0.05, 0. 001 ）. Concentrations of MDA（5.38± 2.76）,Tau （174.97± 12.89） and MDA（4.81 ± 1.54）, Tau（148.61 ± 10.77）in cerebral cortex and gyrus hippocampi of model rats were higher than that of control group （P 〈 0. 001 ）. Concentrations of SOD, GSH- PX, MDA and tau protein showed statistically significant difference between high（750 mg） and moderate dose（500 mg） model group （P 〈 0.05）, and the difference between lower dose group（250 mg） and model group was not signifieant（ P 〉 0.05 ）. Conclusion Codonopsis pilosula （Enshi） can Up - regulate expression of SOD, GSH - PX and down - regulate expression of MDA and tau protein in brain tissues of experimental rats.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2008年第9期1105-1106,共2页
Chinese Journal of Public Health
基金
湖北民族学院生物资源利用与保护(湖北省)重点实验室资助项目(2006-A03)
