摘要
目的研究重组人结缔组织生长因子（rCTGF）对人成骨细胞增殖、分化及凋亡的影响。方法用rCTGF干预体外培养的人成骨细胞，采用[^3H]-TdR掺入法检测人成骨细胞增殖率，α-磷酸萘酚法测定细胞内碱性磷酸酶活性变化，放射免疫法测定骨钙素含量的变化，Western印迹法检测Ⅰ型胶原表达的变化，应用流式细胞仪检测rCTGF对成骨细胞凋亡的影响。结果 rCTGF呈剂量依赖性促进人成骨细胞增殖，200ng／ml rCTGF达最大效应；rCTGF干预显著促进人成骨细胞分化，rCTGF呈剂量依赖性增加Ⅰ型胶原、骨钙素表达及碱性磷酸酶活性；rCTGF干预可显著减少成骨细胞凋亡。结论rCTGF可显著促进人成骨细胞增殖、分化，并抑制人成骨细胞凋亡。
Objective To investigate the effects of recombinant human connective tissue growth factor （rCTC, F） on the proliferation, differentiation and apoptosis in human osteoblasts. Methods Human osteoblasts were treated with rCTGF, cell proliferation was measured by [^3H] 1-thymidine （ [^3H]-TdR） incorporation method, the activity of alkaline phosphatase （ALP） was determined using α-nitrophenyl phosphate as a substrate, the expression of type I collagen was determined by Western blotting, osteocalcin in conditioned media was measured by radioimmune assay, and cell apoptosis was assayed by flow cytometry. Results rCTC, F promoted proliferation and differentiation of human osteoblasts in a dose-depentent manner, rCTC, F increased ALP activity, osteocalcin and type I collagen secretion in a dose-depentent manner, rCTGF inhibited human osteoblastic apoptosis induced by serum deprivation. Conclusion rCTGF can promote osteoblastic proliferation, and differentiation and protect osteoblast against apoptosis, suggesting that CTC, F plays an essential role in bone metabolism.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2008年第4期360-363,共4页
Chinese Journal of Endocrinology and Metabolism
基金
国家自然科学基金资助项目（30572078、30600661）
关键词
结缔组织生长因子
成骨细胞
人
骨质疏松
增殖
细胞凋亡
Connective tissue growth factor
Osteoblasts, human
Osteoporosis
Proliferation
Apoptosis
