摘要
目的观察16Hz，90dB和16Hz，130dB次声对小鼠海马区白介素-6（IL-6）表达及星形胶质细胞胶质纤维酸性蛋白含量（GFAP）的影响，从而探讨次声作用对中枢神经系统自我保护功能的影响。方法共选取BALB／C小鼠60只，将其随机分为90dB次声作用组（20只）、130dB次声作用组（20只）及对照组（20只）。将90dB次声作用组、130dB次声作用组小鼠分别置于次声压力舱内2h，期间分别给予90dB或130dB的次声刺激，对照组小鼠也于同期置人次声压力舱内，但期间不给予次声刺激。于次声作用l，7，14，21及28d时观察各组小鼠海马区IL-6及星形胶质细胞GFAP的表达情况。结果对照组小鼠海马区有一定强度IL-6表达；各次声作用组小鼠海马区IL-6在次声作用7，14及21 d时均较对照组明显增高（P〈0．05），并于次声作用14d时达到峰值；进一步分析后发现，90dB次声作用组IL．6表达水平明显高于130dB次声作用组（P〈0．05）。各次声作用组小鼠海马区GFAP表达水平在次声作用7，14及21 d时均较对照组明显增高（P〈0．05），并于次声作用14d时达到峰值；90dB次声作用组小鼠海马区GFAP表达水平明显低于130dB次声作用组（P〈0．05）。结论次声刺激能显著促进小鼠海马区神经元IL-6表达，提高海马区星形胶质细胞GFAP含量。
Objective To study the effects of infrasound on the expression of interleukin-6 （IL-6） and glial fibrillary acidic protein （GFAP） in the hippocampus of mice. Methods Sixty BALB/C mice were randomized into a 90 dB infrasound exposure group, a 130 dB infrasound exposure group and a control group. The mice of the 2 infrasound exposure groups were exposed to infrasound of 16 Hz at an intensity of 90 dB or 130 dB two hours per day for 1,7,14,21 or 28 days. Immunofluorcscence was used to detect the expression of IL-6 and GFAP in the hippocampus at different time points. Results At 7, 14 and 21 days, IL-6 and GFAP expression in the 2 infrasound exposure groups was significantly increased compared with the control group. At the 14th day of infrasound exposure, the expression of IL-6 and GFAP reached a peak. Moreover, the expression of IL-6 was significantly stronger in the 90 dB infrasound exposure group than that in the 130 dB group, while that of GFAP was significantly lower. Conclusion Infrasound can promote the expression of IL-6 and GFAP in the hippocampus of mice.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2008年第7期452-455,共4页
Chinese Journal of Physical Medicine and Rehabilitation
基金
全军医学科学技术研究、“十五”计划指令性课题（01L071）
关键词
小鼠
海马
白介素-6
星形胶质细胞
GFAP
MOU Xiang, YUAN Hua, JIANG Shan, QU Li-li（Department of Physiotherapy and Rehabilitation, Xijing Hospital, The dth Military Medical University, Xi＇an 710032, China）
