摘要
目的:分析热休克蛋白gp96、髓样细胞白血病-1(MCL-1)和阻抑蛋白(PHB)在LC和HCC组织中的表达及其临床意义.方法:免疫组化二步法分别检测19例LC,32例HCC和21例对照组肝组织中gp96,MCL-1和PHB的表达,并分析其与肝癌临床病理学特征的关系.结果:gp96在对照组、LC组和HCC组的表达逐渐增强(P<0.05);HCC无包膜、有坏死和门静脉有癌栓者gp96表达较高;gp96表达与HCC分化程度呈负相关(r=-0.4655,P=0.0073),与TNM分期呈正相关(r=0.5157,P=0.0025).MCL-1和PHB在HCC组织中过表达,HCC有坏死者MCL-1表达高于无坏死者,HCC无包膜者PHB表达高于有包膜者(P<0.05).结论:gp96,MCL-1和PHB的过表达可能与HCC的发生、发展有关.gp96可能参与LC的发生、发展及向HCC的恶性转化,有助于判断HCC患者的预后.
AIM： To analysis the expression pattern of heat shock protein （HSP） gp96, myeloid cell leukemia sequence-1 （MCL-1） and prohibitin （PHB） in liver cirrhosis （LC） and hepatocellular carcinoma （HCC）, as well as their clinical significances. METHODS： Two-step immunohistochemical method was employed to detect the expression of gp96, MCL-1 and PHB in 19 cases of LC, 32 cases of HCC and 21 controls （non-LC or non- HCC liver tissues） respectively. Their relationships with the clinicopathological characteristics of HCC were investigated.RESULTS： HSP gp96 expression increased by turns in the controls, LC and HCC （P 〈 0.05）, and it was significantly elevated in tumors without envelope, with necrosis or portal venous thrombosis. HSP gp96 expression correlated negatively with tumor differentiation （r = -0.4655, P = 0.0073） and positively with TNM staging （r = 0.5157, P = 0.0025）. Both MCL-1 and PHB were over-expressed in HCC, with the former higher in tumors with necrosis and the latter higher in tumors without envelope than their counterpart （P 〈 0.05）. CONCLUSION： Over-expression of HSP gp96, MCL-1 and PHB may play a role in the genesis and progression of HCC. Gp96 may be implicated in the development of LC as well as its subsequent malignant transformation, and may serve as a prognostic biomarker for HCC.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第23期2661-2665,共5页
World Chinese Journal of Digestology
基金
江苏省卫生厅医学科研项目
No.H200330~~
关键词
肝细胞癌
肝硬化
GP96
髓样细胞白血病-1
阻抑蛋白
免疫组化
Hepatocellular carcinoma
Liver cirrhosis
Heat shock protein gp96
Myeloid cell leukemia sequence-i
Prohibitin, Immunohistochemistry
