摘要
目的:研究不同剂量的放射性125I粒子对家兔尿道的放射性损伤。方法:麻醉下将放射性125I粒子植入雄性家兔尿道旁1.0cm处。125I粒子的放射性粒子活度分别为14.8MBq(A组)、29.6MBq(B组)和44.4MBq(C组),对照组(D组)仅尿道旁种植相当于粒子大小无放射性的无菌铅管1粒。植入后4周,摄尿道片,观察粒子位置等情况;原手术切口切开,取放射粒子周围2.0cm范围内的家兔尿道组织作肉眼、光学显微镜和电子显微镜观察,进行放射性损伤的评价。结果:术后4周,肉眼及光学显微镜观察,实验组与对照组粒子周围的尿道粘膜、粘膜下及肌层所见基本一致;C组少部分电镜视野中观察到尿道上皮胞质出现较多空泡变性、空化、嵴稀疏等超微结构的损伤。光镜下尿道入射性损伤评分,A、B、C、D组分别为(2.20±0.18)、(2.23±0.15)、(2.27±0.10)、(2.10±0.17)分,A、B、C组与D组相比,差异无显著性(P>0.05)。对线粒体作FlaMeng半定量分析,A、B、C、D各组评分分别为(1.23±0.13)、(1.34±0.25)、(1.41±0.30)、(1.12±0.13)分,A、B、C各组与D组(对照组)相比,差异无显著性(P>0.05)。结论:放射性125I粒子对尿道放射性损伤随粒子的放射性活度的增加而逐渐加重,呈明显的放射性活度效应关系;正常剂量的放射性粒子对尿道的损伤是很轻微的,是安全可行的。
Objective： To assess the radioactive damage induced by interstitial ^125I seed implantation to the rabbit urethra. Methods： We implanted 24 rabbits with ^125I seeds 1.0 cm to the urethra at the radiation dose of 14.8 MBq （Group A）, 29.6 MBq （Group B） and 44.4 MBq （Group C） , while a non-radioactive seed was implanted near the urethra of the controls （Group D）. Four weeks later, we detected the radiation-induced pathological and morphological changes in the urethra by H＆E, light microscopy and electron microscopy. Results： Four weeks after the implantation, no obvious histopathological and uhrastructural changes were observed in the urethral tissues of the experimental rabbits as compared with the control group. The scores on the radioactive damages to the urethra obtained by light microscopy were （2.20 ± 0.18）, （2.23 ± 0.15）, （2.27± 0.10） and （2.10 ± 0.17） respectively in Group A, B, C and D, with no significant differences between the first three groups and the control （ P 〉 0.05 ）. And the scores on the FlaMeng semi-quantitative analysis of mitochondria in the experimental groups were （1.23 ± 0.13 ）, （1.34± 0.25 ） and （1.41 ±0.30） respectively, not significantly different from （ 1.12 ± 0.13 ） the control （ P 〉 0.05 ）. Conclusion ： The radioactive damage induced by ^125I seeds to the urethra increases with the enhanced radiation dose. Intraoperative implantation of ^125I seeds at the prescription dose has no obvious adverse effect on the rabbit urethra.
出处
《中华男科学杂志》
CAS
CSCD
2008年第8期709-712,共4页
National Journal of Andrology
