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GHR基因exon 9和exon 10多态性与西农萨能奶山羊产奶性能的相关分析 被引量:3

Polymorphism of exon 9 and exon 10 of GHR gene and its relationship with milk performance of Xinong Saanen dairy goat
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摘要 针对GHR基因exon 9和exon 10设计了2对引物,采用PCR-SSCP技术检测该基因这2个位点在西农萨能羊群上的单核苷酸多态性,同时研究其对产奶性能的影响。结果表明:exon 9具有多态,exon 10不存在多态性。Exon 9位点在西农萨能山羊中检测到NN型和NT型,纯化测序发现有1个SNP位点,NN型与NT型相比在该片段第241 bp处有1个G→T的突变;NN和NT基因型之间产奶量的最小二乘均值差异显著(P<0.05):NN基因型在各胎次产奶量和平均产奶量等指标上均好于NT基因型,其中在第3胎的产奶量和第4胎产奶量2项指标上都达到显著水平(P<0.05)。研究结果提示:GHR基因exon 9对奶山羊产奶量有显著影响,因此认为GHR基因exon 9位点可作为奶山羊高产奶量的标记辅助选择的有效分子标记。 The GHR gene was studied as a candidate gene for milk performance in Xinong Saanen dairy goats. Two pairs of primers were designed to detect single nucleotide polymorphisms of exon 9 and 10 of GHR gene by PCR-SSCP. The results showed that products amplified by exon 10 did not exhibit polymorphisms. For exon 9, two genotypes (NN and NT) were detected in Saanen dairy goats. Sequencing revealed one mutations of the GHR gene in the genotype NN in comparison to the genotype NT; the difference in the least squares means (LSM) for milk yield between NN and NT was significant(P〈0.05). The milk yield of individuals with genotype NN was higher than those with genotype NT in the third and fourth lactation periods (P〈0.05). Based on these results, the GHR gene had significant positive effects on milk performance of dairy goats and the exon 9 of the GHR gene may be regarded as an effective molecular marker on the MAS program for the milk performance of dairy goats.
出处 《中国农业大学学报》 CAS CSCD 北大核心 2008年第5期70-74,共5页 Journal of China Agricultural University
基金 国家"863"资助项目(2007AA10Z167) 国家"十一五"奶业重大科技支撑项目(2006BAD04A11)
关键词 西农萨能奶山羊 PCR—SSCP GHR基因 产奶性能 EXON Saanen dairy goat PCR-SSCP GHR milk performance exon
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  • 1Finbloom D S, Petricoin E F, Hackett R H, et al. Growth hormone and erythropoietin differention ally activate DNA-binding proteins by tyrosine phosphorylation[J] . J Mol CellBio,1994,14:2113-2118
  • 2Georges M, Nielsen D, Mackinnon M A , et al. Map ping quantitative trait loci controlling milk production by exploiting progeny testing [J]. J Genetics, 1995, 139:907-920
  • 3Arranz J J, Coppieters W, Berzi P, et al. A QTL affecting milk yield and composition maps [J]. Theor Appl Genet, 1998,93:71-80
  • 4郑丕留.中国羊品种志[M].上海:上海科学技术出版社,1988..
  • 5Ji Yun-tao, Qu Chang-qing, Cao Bin-yun. Optimized method of DNA silver staining in polyacyla-mide gels electrophoresis [J]. Electrophoresis, 2007, 28: 1173- 1175
  • 6Falaki B, Gengler M N, Sneyers M, et al. Relationships of polymorphisms for growth hormone and growth hormone receptor genes with milk production traits for Italian Holstein-Friesian bulls [J]. J Dairy Sci, 1996,79: 1446-1453
  • 7Moisio S K, Kantanen E J, Vilkki J. Polymorphism within the 3r flanking region of the bovine growth hormone receptor gene[J]. J Anim Genet, 1998,29: 55-57
  • 8Ge W, Davis M E, Hines H C, et al. Two-allelic DGGE polymorphism detected in the promoter region of the bovine GHR gene[J]. J Anim Genet, 1999,30: 71
  • 9Ge W, Davis M E, Hines H C, et al. Rapid Communication: Single nucleotide polymorphisms detected in exon 10 of the bovine growth hormone receptor gene [J]. J AnimSci, 2000,78:2229-2230
  • 10高雪,许尚忠,张英汉.牛生长激素受体基因遗传变异研究[J].中国农学通报,2005,21(8):10-12. 被引量:12

二级参考文献30

  • 1李吉涛,杜立新.中国荷斯坦牛催乳素基因型与产奶性状的相关分析[J].山东农业大学学报(自然科学版),2004,35(4):553-555. 被引量:21
  • 2Finbloom D S, Petricoin E F, Hackett R. H, et al. Growth hormone and erythropoietin differentially activate DNA-binding proteins by tyrosine phosphorylation J. Mol. Cell. Bio, 1994,14:2113-2118
  • 3Georges M., Nielsen D, Mackinnon M., A. et al. Mapping quantitative trait loci controlling milk production by exploiting progeny testing. J.Genetics, 1995,139:907-920
  • 4Arranz J J, Coppieters W, Berzi P, et al. A QTL affecting milk yield and composition maps.J. Theor. Appl. Genet., 1998,93:71-80
  • 5Falaki B, Gengler M. N, Sneyers M., et al. Relationshipsof polymorphisms for growth hormone and growth hormonereceptor genes with milk production traits for Italian Holstein-Friesian bulls. J. Dairy Sci,1996,79:1446-1453
  • 6Moisio S K, Kantanen E J, Vilkki J. Polymorphismwithin the 3'flanking region of the bovine growth hormone receptor gene.J. Anim.Genet, 1998,29:55-57
  • 7Ge W, Davis M. E, Hines H C, et al.. Two-allelic DGGE polymorphism detected in the promoter region of the bovine GHR gene. J. Anim.Genet., 1999,30:71
  • 8Ge W, Davis M E, Hines H C, et al.Rapid Communication: Single nucleotide polymorphisms detected inexon 10 of the bovine growth hormone receptor gene. J. Anim. Sci., 2000,78:2229-2230
  • 9Hale C S, Herring W O, Shibuya H, et al. Decrease growth in Angus steers, with TG-microsatellite allele in P1 promoter of the growth hormone receptor gene. J. Animal Science, 2000,78:2099-2104
  • 10Aggrey S E, Yao J, Sabour M P, et al. Markers within the regulatory region of the growth hormone receptor gene and their association with milk-related traits in Holsteins. J. Heredity, 1999,90(1):148-151

共引文献34

同被引文献36

  • 1徐秀容,高雪,许尚忠,张英汉,任红艳,李俊雅.牛DGAT2基因第6内含子MspI-RFLPs和TaqI-RFLPs及其与牛经济性状相关性研究[J].畜牧兽医学报,2005,36(10):981-986. 被引量:14
  • 2季香,马月辉,叶绍辉,李菜娥.反刍动物生长激素受体基因的研究进展[J].草食家畜,2006(2):27-31. 被引量:10
  • 3Lardizabal K D, Mai J T,Wagner N W,et al. DGAT2 is a new diacylglycerol acylt ransferase gene family[J]. J Biol Chem,2001,276(42) :38862-38869.
  • 4Man W C, Miyazaki M, Chu K, et al. Co-localization of SCD1 and DGAT'2:implying preference for endogenous monounsaturated fatty acids in triglyceride synthesis[J], J Lipid Res, 2006,47(9) : 1928-1939.
  • 5Casaschi A, Maiyoh G K,Adeli. Increased diacylglycerol acyltransferase activity is associated with triglyceride accumulation in tissues of diet-induced insulin-resistant hyperlipidemic hamsters[J]. Metabolism, 2005,54 (3) 403-409.
  • 6Buhman K K,Smith S J,Stone S J. DGAT1 is not essential for intestinal triacylglycerol absorption or chylomicron synthesis[J]. J Biol Chem, 2002,277 (28):25474-25479.
  • 7Winter A, Van E M, Bininda-emonds O R, et al. Genomic organization of the DGA72/MOGAT gene family in cattle (Bos taurus) and other mammals[J]. Cytogenet Genome Res, 2003,102 (1/2/3/4) : 42-47.
  • 8Chen H, Leibenguth F. Studies on multilucos fingerprints,RAPD markers and mitochondrial DNA of a gynogenetic fish[J]. Biochem Genet, 1995,33 (9/10) : 297-306.
  • 9Liu B H. Statistical Genomics: Linkage, Mapping, and QTL Analysis[M]. LLC: CRC Press, 1998 : 404-409.
  • 10Stone S J,Myers H M,Watkins S M,et al. Lipopenia and skin barrier abnormalities in DGAT2 deficient mice[J]. J Biol Chem,2004,279(12) : 11767-11776.

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