摘要
双孢蘑菇母种培养基的优化首先采用单因素试验所得到的最佳碳源、氮源和维生素的种类以及最适合的无机盐含量和pH,并进一步通过正交试验确定了最佳配方为玉米粉4%、蔗糖2%、蛋白胨0.1%、硫酸镁0.25%、磷酸二氢钾0.4%、维生素B1 0.05%、pH 6.0。
Optimum carbon source, nitrogen source, vitamin and inorganic salt were decided for the medium of the stock spawn of Agaricus bisporus by the single factor experiment. Furthermore, the optimum formula of medium for stock spawn was decided eventually by orthogonal experiment, which was 4% corn, 2% sucrose, 0.1% peptone, 0.4% KH2PO4, 0.25% MgSO4, 0.05% VB1, pH 6.0.
出处
《东北农业大学学报》
CAS
CSCD
2008年第8期70-73,共4页
Journal of Northeast Agricultural University
基金
哈尔滨市科技攻关计划项目(GJ2007GG002522)
东北农业大学创新团队发展计划项目(CXT003-2-1)
关键词
双孢蘑菇
母种
培养基
玉米粉
Agaricus bisporus
stock spawn
medium
corn
