摘要
目的:探究微量元素(铜、锌)对丹参根中3种丹参酮的累积产生影响的机制,为丹参的优质高产提供理论依据。方法:通过砂培试验研究铜、锌对丹参根中3种丹参酮的累积和丹参体内过氧化物酶(POD)、多酚氧化酶(PPO)活性的影响,并且探究了它们与基质中有效态铜、锌含量之间的相关关系。结果:丹参根中3种丹参酮的含量均随铜、锌浓度的增大而提高,对丹参生长期内3种丹参酮的动态监测表明,铜、锌处理后60 d时的3种丹参酮含量最低,120 d时最高,150 d时又开始下降。在基质中有效态铜和有效态锌含量、丹参体内氧化酶(POD,PPO)活性、丹参根中3种丹参酮含量三者之中,任何两者之间均呈现显著(P<0.05)或极显著(P<0.01)的正相关关系。结论:铜、锌对丹参根中丹参酮类物质累积产生影响的机制有可能是适量的铜、锌增强了多酚氧化酶、过氧化物酶的活性,而他们又有可能促使了丹参体内酚类物质更多的转化成萜类物质,从而使丹参酮的累积量增加。
Objective： To study the mechanism of microelement including Cu and Zn on the accumulation of three danshinones in Salviae miltiorrhizae root and build a theory basis for its good quality and high yield. Method： Sand culture experiments were conducted to study the effect of Cu and Zn on the accumulation of three danshinones and oxidase including peroxidase and polyphenol oxi- dase activity in the plant root. The correlation between available Cu and Zn contents in matrix and oxidase activity in the plant root and, the correlation between available Cu and Zn contents in matrix and contents of tanshinones in the root were discussed. Result： Contents of danshinones in the root increased with the increasing of Cu and Zn concentration. Dynamic monitoring on contents of danshinones of the plant roots growing in the pots with different Cu and Zn concentration in the whole growing season showed that the contents of danshinones for 60 days were the lowest, for 120 days the highest and then dropped for 150 days. In addition, among available Cu and Zn contents of matrix, oxidase including peroxidase and polyphenol oxidase activity and contents of tanshinones in the root, the correlation between two factors were significant difference（P 〈 0.05 or P 〈 0.01 ）. Conclusion： The mechanism of Cu and Zn on the accumulation of danshinones may be that Cu and Zn improve the activity of peroxidase and polyphenol oxidase, which promote transformation of phenolic compounds to terpenes and therefore to increase contents of danshinones.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第17期2082-2087,共6页
China Journal of Chinese Materia Medica
基金
国家科技支撑计划项目(2006BAI09B03)
