摘要
采用纤维素酶-微波联合提取虎杖中的白藜芦醇。利用正交试验分别优化白藜芦醇的酶法提取工艺和微波提取工艺。酶法辅助提取白藜芦醇的适宜提取条件为:酶解温度为50℃、pH值为5.0、纤维素酶与虎杖粗粉质量配比为1∶500、酶解时间为90 min,比传统乙醇提取法提取收率提高2倍;微波辅助醇提白藜芦醇的适宜提取条件为:以体积分数为80%的乙醇溶液为提取剂,料液质量比为1∶20,在510 W功率下微波1.0 min,比传统乙醇提取法提取收率提高近3倍;利用酶微波结合提取白藜芦醇,酶解30 min,510 W功率下微波10 s,提取收率提高3倍,缩短了提取时间,降低了生产成本。纤维素酶酶解后进行微波处理的工艺路线可有效提取虎杖中白藜芦醇。
To improve the extracting ratio of resveratrol from giant knotweed, a new process was developed in this paper by using cellulose and microwave. The optimum conditions of enzyme extraction and microwave extraction were obtained through orthogonal test. For the enzyme extraction, with the ratio of enzyme/giant knotweed 1：500 at 50 ℃ for 90 min, the yield of resveratrol is two times more than that extracted by ethanol. For the microwave extraction with the ethanol content of 80%, the liquid ratio of 1：20, extracting by microwave for 60 s at 510 W, the yield of resveratrol is three times more than that extracted by ethanol. For the enzyme-microwave extraction, the yield of resveratrol is three times more than that extracted by ethanol with enzymation 30 min by microwave for 10 s at 510 W. So the enzyme-microwave extraction can shorten the extraction time and lower the cost of production, it is an effective way to extract resveratrol from giant knotweed .
出处
《化学工业与工程》
CAS
2008年第5期394-398,共5页
Chemical Industry and Engineering
关键词
虎杖
白藜芦醇
纤维素酶
微波
正交试验
giant knotweed
resveratrol
cellulose
microwave
orthogonal test
