摘要
在Ｇｕｔｏｎ１９６６年创立的流导测定法基础上，我们创立了一种新方法。用压差传感器代替Ｇｕｙｔｏｎ实验中的弹性腔和压力传感器产生压力差并直接转换成电信号输入计算机，使用两个ＷＩＮ针头作测量，通过对数转换和线性回归计算出流导率。在麻醉的大白鼠和小型猪活体的循经低电阻线皮下组织和对照区上进行了测量，分别使用２．５ｃｍＨ２Ｏ和１０ｃｍＨ２Ｏ两种压力差。结果表明，大白鼠经脉和非经脉组织的流导率分别为１４．５±１５．９２和９．５５±１４，０１（×１０－８，ｃｍ４／ｄｙｎｅ－ｓｅｃ），小型猪经脉组织和非经脉组织的流导率分别为１，７２±２．１９和０．７８±３．１７（×１０－５；ｃｍ４／ｄｙｎｅ－ｓｅｃ），经脉上的流导率比经脉外显著高（Ｐ＜０．０５）。本方法可消除同步干扰，两种压力差下测得的流导率数值与Ｇｕｙｔｏｎ在非水肿和水肿两种状态下测得的流导率基本相同，说明了间隙组织流导的生物流变特性。
On the basis of Guyton' s method of measuring hydraulic conductance(G), we developed a new method. Guyton's chambers with membrane and pressure transducers were replaced by a differential pressure transducer, which produced a pressure difference (PD). The PD was changed into voltage and sent to a computer two needles with side holes were used to measure G. The data was transformed logarithmically and linear recurreive calculation was used to obtain hydraulic conductivity(HC). We measured the HC in meridian tissue and control area subcutaneously on anesthetized rats with PD of 2.5 cmH2O and mini - pigs with PD of 10cmH2O respectively. The results showed that the HC in meridian and control area were 14.5± 15.9 and 9.55±14.0l( x 10'-8, cm4/ dyne-sec) respectively in rats, the HC in meridian and control area were 1.72± 2.19 and 0.78± 3. l7( x 1 0 - 5, cm4 / dyne-sec) respectively in mini-pigs. The HC in meridian tissue were significantly higher both in rats and mini-pigs (P<0.05). The method can eliminate synchronous interferences. The HC, measured with two PD, corresponded basically to the data measured by Guyton in the cases of nonedematous and edematous, which showed the biorheological feature of the interstitial tissue.
出处
《北京生物医学工程》
1997年第4期199-204,共6页
Beijing Biomedical Engineering
基金
国家自然科学基金
关键词
流导
经脉
压差传感器
Hydraulic conductance
Meridian
Differential pressure tuansducer,Rat
Mini-pig
