摘要
目的:应用蛋白质芯片技术平台研究慢性重型肝炎血清中的低分子量蛋白质。方法:利用表面增强激光解吸离子化飞行时间串联质谱技术,检测28例慢性重型肝炎患者和22例健康对照组血清中相对分子质量位于1 kD-10 kD之间的蛋白质指纹图谱,筛选两组图谱的差异峰,结合主成分分析方法区分健康对照组和重型肝炎患者;对慢性重型肝炎组和健康对照组的血清样本进行超滤处理,研究超滤前后血清蛋白质指纹图谱的变化情况。结果:比较慢性重型肝炎组与健康对照组的蛋白质指纹图谱,有39个峰强度在两组间的P<1×10-6。用主成分分析方法对得到的数据进行处理,在得到的投影结果图上,健康对照组与慢性重型肝炎组的划分边界非常清楚。对比血清超滤前后的蛋白质指纹图谱,发现在经过截留率为30 kD的超滤膜超滤后,超滤液中相对分子质量位于1 kD^10 kD的峰基本消失。结论:慢性重型肝炎患者的血清中存在具有明显差异的低分子量蛋白质。
AIM： To profile the serum low molecular weight proteins in patients with chronic severe hepatitis with proteinchip technique. METHODS： 28 cases of chronic severe hepatitis and 22 healthy controls were enrolled in the study. Serum samples were analyzed with the surface enhanced laser desorption ionization - time of flight - mass spectrometry （ SELDI - TOF MS） to obtain a quantitative proteomic fingerprints with molecular masses ranging from 1 kD to 10 kD. The discriminating peaks of two groups were identified. Principal component analysis was then used as the classification method for modeling the discrimination between chronic severe hepatitis patients and healthy controls. Serum was subjected to centrifugal uhrafihration, and the unfiltered serum and the uhrafihrates were compared using SELDI - TOF MS. RE. SULTS： Compared the proteomic fingerprints of severe hepatitis serum to control serum, 39 discriminating peaks were identified （ P 〈 1 × 10^-6 ）. Application of principal component analysis to SELDI - TOF MS data distinguished control samples from severe hepatitis samples clearly. Compared with the unfiltered serum, the SELDI - TOF MS spectrum of the filtered serum showed very few peaks with molecular masses ranging from 1 kD to 10 kD. CONCLUSION ： Differentially expressed low molecular weight proteins are observed in the serum of chronic severe hepatitis patients. On the basis of the relevant literature, the biological significance of the present study is discussed.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2008年第9期1794-1798,共5页
Chinese Journal of Pathophysiology
基金
国家863计划资助项目(No.2006AA02A108)
关键词
光谱分析
质量
肝炎
蛋白质组
Spectrum analysis, mass
Hepatitis
Proteome
