摘要
目的探讨食管和贲门癌及癌前病变患者血清中cyclinB1、p62、Koc、IMP1和Survivin自身抗体检测及其在癌组织中表达变化特征。方法应用间接酶联免疫反应法和肿瘤相关抗原微阵列（包含5个重组的癌抗原蛋白cyclinB1、p62、Koc、IMP1和Survivin）检测376例食管和贲门各级病变患者（正常、癌前病变、癌）血清中的自身抗体；同时应用免疫组化ABC法检测与血清对应的13例食管癌和16例贲门癌组织中上述5个相关抗原。结果在5个抗原中，虽然cyclinB1、IMP1和p62从正常食管到癌前病变、癌患者血清中和p62在正常贲门到癌前病变、癌患者血清中阳性百分率均具有线性升高趋势（P〈0，05），但单一抗体对食管和贲门癌的检出率均较低，而当应用多个抗原分析时，即在5个肿瘤相关抗原中至少有一个反应为阳性时，与单一抗体比较食管癌和贲门癌的阳性检出率分别提高了3～5倍／3～4倍，并且，癌患者和食管、贲门各级病变患者血清阳性表达率差异显著（P〈0．05）。在癌组织中，5个抗原均有阳性表达，cyclinB1、Koc、IMP1和Survivin阳性率均明显高于血清抗体的检出率（P〈0.05），血清中抗体阳性的抗原在同一患者癌组织中均可测得。结论肿瘤自身抗体的产生与相关抗原的表达有关，联合应用多个肿瘤相关抗原比应用单个肿瘤相关抗原分析食管和贲门癌及癌前病变患者血清中的自身抗体变化更能提高癌和癌前病变患者的检出率。
Objective To characterize the alterations of serum autoantibodies for cyclinB1 ,p62, Koc,IMP1 and survivin in the subjects with esophageal and gastric cardia carcinoma and precancerous lesion and their expressions in the esophageal and gastric cardia cancer tissue. Methods Enzyme-linked immunoassay and tumor-associated antigen mini-array （ consisting of five full-length recombinant proteins, including cyclinB1, p62, Koc, IMP1 and Survivin） were applied to determine the serum level of the autoantibodies of these antigens on 376 subjects with esophageal and gastric cardia carcinoma and precancerous lesions. At the same time, the expression of these antigens was detected by immunohistochemical method （ABC） on 13 patients with esophageal cancer and 16 with gastric cardia cancer. Results All of the 5 antigens determined, the linear correlation was observed for the detection frequency of cyclinB1 ,IMP1 and p62 in esophageal carcinogenesis, and for p62 in gastric cardia multi-stage progression from normal to precancerous and cancerous lesions （ P 〈 0.05 ）. The detection rate with single positive autoantibody immunoreactivitv for both esoohageal and gastric cardia cancers were low. However, the positive detection rate for both esophageal and gastric cardia cancer increased apparently when the multiple positive markers were combined together for analysis,which increased to 3 -5 and 3 -4 folds respectively. Furthermore,the difference in autoantibody immunoreactive rate was significant with the lesion progressed from mild to severe precancerous lesions and to cancer both in esophageal and gastric cardia cancers （ P 〈0.05 ）. The positive immunoreactions of the 5 antigens were detected in cancer tissues. The positive immunostaining rates for cyclinBl, Koc, IMP1 and Survivin both in esophageal and gastric cardia cancers were higher compared to their serum positive rate of autoantibodies （ P 〈 0.05 ）. Of the patients with positive immunostaining in the two cancer tissues,the autoantibodies in the serum for the corresponding antigens could be detected in the same patient. Conclusion The production of the tumor-associated autoantibodies is related to antigens. The screening rate through serum tumor-associated antigen mini-array for the patients with esophageal and gastric cardia carcinoma and precancerous lesions has been increased apparently with combined analysis of multiple autoantibodies than with single one.
出处
《中国综合临床》
北大核心
2008年第10期970-974,共5页
Clinical Medicine of China
基金
国家自然科学基金项目（30572115）
国家杰出青年科学基金项目（30025016）
河南省食管癌重点开放实验室基金项目（20050227）
河南省医学科技攻关项目（20058）
河南省高校创新人才工程基金项目（1999125）
郑州大学211工程项目
关键词
食管癌
贲门癌
肿瘤相关抗原
自身抗体
Esophageal carcinoma
Carcinoma of gastric cardia
Tumor-associated antigens
Autoantibody
