摘要
目的运用噻唑蓝(MTT)法检测中药益气养阴方及其拆方后的扶正组方、祛邪组方对人白血病CD34+CD38-干细胞群的影响。方法免疫磁珠分离人白血病CD34+CD38-干细胞群,MTT法检测中药复方对细胞生长的影响。结果益气养阴制剂对白血病干细胞的体外抑制率为(24.390±0.046)%,扶正组抑制率为(9.270±0.052)%,祛邪组为(9.370±0.017)%,益气养阴组与扶正组、祛邪组比较差异均有统计学意义(P<0.05),扶正组与祛邪阻比较差异无统计学意义。结论益气养阴方对白血病干细胞有明显抑制作用。
Objective To determine the response of human CD34^＋/CD38^- leukemic stem cell （LSCs） to Yiqi Yangyin Fang, a traditional Chinese formula, used as a full package or part of its supportive or aggressive components. Methods Magnetic activated cell sorting （MACS） was used to select CD34^＋/CD38^- LSCs. The growth of these cells in response to TCM recipes was determined with MTF assay. Results The growth inhibition was found in （24.390±0.046）% of LSCs exposed to full-package Yiqi Yangyin Fang, compared with （9.270±0.052）% of those exposed to the supportive components and （9.370±0.017）% exposed to aggressive components. CD34^＋/CD38^- LSCs were more susceptible to the full-package than to either of its components （P〈0.05） which did not show difference between each other. Conclusion The Yiqi Yangyin formula exhibited remarkable inhibitory effects on leukemic stem cells.
出处
《中国药物与临床》
CAS
2008年第9期696-698,共3页
Chinese Remedies & Clinics
基金
国家自然科学基金资助项目(30672746)
