摘要
背景:应用涤纶连接环的设计有效地解决了人工食管研制中发生早期吻合口漏和脱管的难题,实验动物均能长期存活。但带涤纶连接环记忆合金组合式人工食管还存在中晚期脱管的问题未能完全解决。目的:观察改变镍钛合金组合式人工食管的管体结构在防止脱管中的作用。设计、时间及地点:对比观察动物实验,于2007-09/2008-05在广州医学院第二附属医院动物实验室完成。材料:健康小型实验香猪6只由广东省医学实验动物中心提供。带涤沦连接环编织型外层裸管体双层结构镍钛合金组合式人工食管和带涤沦连接环网格型覆膜单管结构镍钛合金组合式人工食管均由北京有色金属研究学院制作提供。方法:通过手术切除一段长约7cm的胸段食管,应用编织型和网格型两种不同管体结构的镍钛合金组合式人工食管替代胸段食管重建食管通道的方法,建立两组实验动物模型,每组3只。主要观察指标:人工食管的位置,新生食管与植入体的结合状况。结果:实验动物均存活超过3个月。网格型组3只(100%)术后3个月内全部出现脱管,脱管发生时间60～68d(平均63d)。编织型组3只(100%)术后3个月内无发生脱管,远近端正常食管无扩张及狭窄,新生食管中间段部分管壁组织向植入体网孔内长入紧密固定。结论:编织型镍钛合金组合式人工食管替代胸段食管后,成功诱导新生食管与管体紧密结合,提示改变镍钛合金组合式人工食管管体结构是防止脱管的有效方法,同时也是解决永久性人工食管脱管这一难题的有效途径。
BACKGROUND： Terylene tandem ring effectively solves the difficulties of early stomal leak and trachea cannula exodus during artificial oesophagus development. Experimental animals can live for a long time. However, trachea cannula exodus of terylene tandem ring alloy composite artificial esophagus in middle and late phases cannot be fully solved. OBJECTIVE： To explore the role of the tube structure changes in Nitinol alloy composite artificial esophagus to prevent trachea cannula exodus. DESIGN, TIME AND SETTING： The control observation animal experiment was performed at the Animal Laboratory of Second Affiliated Hospital, Guangzhou Medical College from September 2007 to May 2008. MATERIALS： Six healthy mini-pigs were obtained from Medical Animal Experimental Center of Guangdong Province. Weaving type of the nitinol composite artificial esophagus with polyester connecting ring and grid type of the nitinol composite artificial esophagus with polyester connecting ring were obtained from General Research Institute for Nonferrous Metals. METHODS： The models of experimental animal were established by excised a segment of 7-cm long thoracic esophagus and replaced with the two different tube structure of Nitinol alloy composite artificial esophagus, which were divided into the grid type group and the weaving type group, three models in either group. MAIN OUTCOME MEASURES： Location of artificial esophagus and the united situation between neo-esophagus and the implantation artificial esophagus. RESULTS： Experimental animals lived for over 3 months. Grafted prostheses exodus took place in three （100%） of the grid type group from a time of 60-68 days （an average of 63 days）. None of the models in the weaving type group developed exodus. Distal and proximal esophagus was not expanded and narrow. Middle neo-esophagus grew towards implants tightly. CONCLUSION： The braided nitinol modular structure of artificial esophageal replacement of thoracic esophagus can be the successful induction of new esophagus and the close integration, suggesting that changes in nickel titanium alloy modular structure of the esophagus is the effective methods to prevent the grafted prostheses from sloughed out. And also to be the effective ways resolved to take place the grafted prostheses sloughed out in permanent artificial esophagus.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第35期6806-6810,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
广东省科技厅科技计划项目(2004B10401036)~~
