摘要
采用高效液相色谱-电喷雾串联四极杆质谱(HPLC-ESI-MS/MS)在选择反应监测(SRM)模式下分离鉴定纺织品中禁用的9种致癌染料。用甲醇超声同时提取天然纤维和化学纤维上的染料,以5mmol/L乙酸铵和乙腈为流动相在C18柱上于前段洗脱酸性红26、直接蓝6、直接黑38和直接红28(采用电喷雾质谱负离子模式检测),于后段洗脱碱性红9、碱性紫14、分散蓝1、分散橙11和分散黄3(采用电喷雾质谱正离子模式检测),实现了对不同种类纤维织品中分属4类性质不同染料的一次提取和一次分析检测。通过比较试样与标样的色谱保留时间和质谱图中子离子的相对丰度比,可准确鉴别纺织品中的致癌染料。
A qualitative method of the identification of nine carcinogenic dyes prohibited in textiles was developed using high performance liquid chromatography-tandem mass spectrometry interfaced with electro-spray ionization（HPLC-ESI-MS/MS）in the selective reaction monitoring（SRM）mode.The dyes were extracted from textiles composed of natural or chemical fibers by methanol under ultrasounication,and then eluted with gradient by acetonitrile and 5 mmol/L ammonium acetate from an RP-C18 column with two segments in effluents.The first effluents accommodated Acid Red 26,Direct Blue 6,Direct Black 38 and Direct Red 28 with negative ionization mode,while the second accommodated Basic Red 9,Basic Violet 14,Disperse Blue 1,Disperse Orange 11 and Disperse Yellow 3 with positive ionization mode.Thus the investigated compounds could be identified simultaneously with single-run analysis no matter which type of the fibre the sample was and no matter which category of the dye the analyte was.The established method was successfully applied to identify the carcinogenic dyes in textile samples through comparing the chromatographic retention time and the relative abundance of characteristic product ions with the standards.
出处
《色谱》
CAS
CSCD
北大核心
2008年第5期603-607,共5页
Chinese Journal of Chromatography
基金
"十一五"国家科技支撑计划重点项目(2006BAK10B03-3)
关键词
高效液相色谱-串联质谱
致癌染料
纺织品
high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）
carcinogenic dyes
textile
