摘要
细胞运动特征及其变化主要受细胞自身状况和微环境二方面的影响,细胞适应不同环境的运动响应方式存在差异,在二维培养基质上细胞迁移方式主要分为个体迁移和群体迁移,而在三维培养基质中其迁移模式主要为间充质迁移和阿米巴迁移。肿瘤细胞因其结构功能状况异常,在上述环境中的迁移特征出现不同程度的异化,其主要倾向为顽固、无目的和侵袭性的迁移运动。对细胞的迁移能力进行量化表征,有助于对细胞迁移本质的进一步认识。根据细胞培养环境的不同,分别介绍了二维和三维培养基质上细胞的不同迁移模式及肿瘤细胞的迁移运动特征,以及测量细胞迁移能力的体外测试手段和方法,并分析总结了这些方法的优缺点。
Characteristics and changes in cell motility are affected by the condition of cells as well as micro- environment, which influced cells＇ movement behavior to adjust to the different micro-environment. In the two- dimensional culture matrix, cell migration patterns can be divided as single migration or collective migration, and in three-dimensional cultivate their migration patterns cell undergo mesenchymal migration or amebic migration. Because of the abnormal conditions of structure and function, the cancer cell moving characteristics is in varying degrees of alienation contrast to normal cell. Quantify the abilities of cell migration contribute to the further understanding of nature. Based on the different cell culture environment, we introduced the two-dimensional and threedimensional culture matrix cell migration patterns, stated the characteristics of tumor cell migration and movement, as well as the testing means and methods to measure cell migration in vitro, and we also analyze the pros and cons of the in vitro tests available to quantify quantitative of cell migration.
出处
《细胞生物学杂志》
CSCD
2008年第4期451-456,共6页
Chinese Journal of Cell Biology
基金
国家自然科学基金资助项目(No10372137)~~
关键词
细胞迁移模式
迁移能力量化
肿瘤细胞运动特征
mode of cell migration
quantitative analysis of cell migration
characteristics of cancer cell migration
