摘要
合成了N,N’-双(2-羟基-1-萘酚醛)-2,6-二亚胺吡啶配体(L)及其Co(Ⅱ)配合物.通过元素分析、摩尔电导率、红外吸收光谱、紫外吸收光谱及差热-热重分析,确定了配合物的组成为Co(NO3)2L.2H2O.在pH值为7.25的Tris-HCl缓冲溶液中,以溴化乙锭为荧光探针,用荧光光谱、黏度实验测定研究了Co(NO3)2L.2H2O与ct-DNA的相互作用,结果表明,Co(NO3)2L.2H2O与ct-DNA容易发生插入作用,结合常数Ka=2.34×105L/mol,结合位点为1.
A novel lig complex were syn and of N, N＇-bis （2-hydroxy-l-naphthaldehyde）-2,6-pyridylenediimine （L） and its Co （Ⅱ） esized. Co（ Ⅱ ） complex was characterized by elementary analysis, molar conductivity, IR, UV and thermal analysis. The results suggested that the possible composition of the complex was Co（NO3）2 L · 2H2O. The mechanism of interaction between Co（ Ⅱ ） complex and calf thymus ct-DNA has been studied with ethidium bromide （EB） as a spectral probe by spectrophotometry and viscosity measurement in buffer solution of Tris-HCl（pH=7.25）. All of the results indicated that the interaction of the Co（ Ⅱ ） complex with ct-DNA is intercalation, the binding constant （Ka） is 2.34×10^5 L/mol, and the binding spot（n） is 1.
出处
《宁夏大学学报（自然科学版）》
CAS
北大核心
2008年第3期244-248,共5页
Journal of Ningxia University(Natural Science Edition)
基金
教育部科学技术研究重点基金资助项目(208159)
宁夏高等学校科研基金资助项目
