摘要
[目的]探讨急性毒性试验替代法之一—上-下法的优、缺点,寻找最佳的实验条件及具体实施过程中可能存在的问题。[方法]采用上-下法与Horn’s法分别测定11种农药制剂急性经口LD50和95%可信区间(CI),比较两种方法获得的急性毒性分级和试验所用动物数量。[结果]上-下法所得LD50相近,毒性分级与Horn’s法基本一致;但上.下法试验使用动物减少了64.7%。[结论]上-下法在测定毒作用发生在48h内的农药急性经口LD50具有明显优势,在国内是值得推广的急性替代方法之一。
[Objective] To discuss the advantage and disadvantage of the up-and-down procedure, and to search the best condition and possible problems of practice. [ Methods] The median lethal dose （LD50） and 95% confidence intervals of 11 pesticides were detected with the advantage and disadvantage of the up-and-down procedure, and the two acute toxicity classifications and the animal consumption of the two method were compared. [Results] The LD50 and 95% confidence intervals with the Up-and-Down Procedure are similar with the horn＇s, while the animals could be economized by 64.7% with the Up- and-Down Procedure when compared with the hern＇s. [Conclusion] The Up-and-Down Procedure has the advantage of being a substitute for determining the LD50 of pesticides after oral if the toxic effects occur within 48 hours, which is worthy of popularization.
出处
《现代预防医学》
CAS
北大核心
2008年第18期3591-3593,共3页
Modern Preventive Medicine
