摘要
目的研究维药伊木萨克片对DM性ED大鼠阴茎海绵体组织中eNOS和nNOS蛋白表达水平的影响。方法取雄性SD大鼠70只,从中随机取10只为正常对照组,余60只以链脲佐菌素诱导建立DM模型后,行阿朴吗啡阴茎勃起实验筛选DM性ED模型,发生ED者随机分为DM性ED组、伊木萨克片组、胰岛素组、伊木萨克片+胰岛素(联用)组,未成DM者为STZ组。各组给药6周后,采用免疫组化SP法检测各组大鼠阴茎组织中eNOS、nNOS含量。结果DM性ED组大鼠阴茎组织中eNOS、nNOS蛋白表达水平显著低于正常对照组(P<0.01);伊木萨克片组、胰岛素组及联用组大鼠阴茎组织中eNOS、nNOS蛋白表达水平均显著高于DM性ED组(P<0.01),但伊木萨克片组与胰岛素组显著低于联用组(P<0.01,P<0.01;P<0.05,P<0.01);STZ组与正常对照组之间的差异则无统计学意义(P>0.05)。结论维药伊木萨克片可以显著提高DM性ED大鼠阴茎组织中eNOS、nNOS蛋白表达水平,并提示在胰岛素控制血糖的基础上效果可能更佳。
Objective To study the effect of the Yimusake tablet on the expression of nNOS and eNOS in the corpus cavernosum tissue of erectile dysfunction diabetic rat. Methods Seventy male sprague-dwaley rats were used in this study. Sixty rats of them with normal erectile function were injected with streptozotozin for estabilishment of diabetes mellitus（DM） models. The other ten nomral rats were enrolled in the normal group. All of the DM rats were injected with apomorphine to test their erectile function. The rats injected with streptozotozin were divided into five groups including DMED group, Yimusake group, insulin group, Yimusake with insulin group, the rats with no-diabetes were called the STZ group. The expression of eNOS and nNOS .were detected by means of SP immunohistochemistry in the corpus cavernosum tissue of rats of each group after six weeks treatment. Results The expression of eNOS and nNOS in DMED group were significantly lower than that in the control group （P〈0.01, P〈0.01）; And the expression of eNOS and nNOS in the Yimusake group, the insulin group and the Yimusake with insulin group were higher＇than that in DMED group（P〈0.01）. However, the expression of eNOS and nNOS in the Yimusake group and the insulin group were lower than that in the the Yimusake with insulin group（P〈0.05, P〈0.01）. There was no significant difference in the expression of nNOS and eNOS between the STZ group and the normal group （P〉0.05）. Condusion Yimusake tablet can significantly stimulated the expression of eNOS and nNOS in the corpus cavernosum tissue of erectile dysfunction diabetic rat,and indicated that it might have a better effect on erectile dysfunction under the controlling blood sugar with insulin.
出处
《中国男科学杂志》
CAS
CSCD
2008年第8期10-15,共6页
Chinese Journal of Andrology
