摘要
目的:研究黄芪皂苷(AS)增强巨噬细胞的免疫作用,探讨其调节机体免疫功能的机理。方法:在体外培养的小鼠腹腔巨噬细胞中加入不同浓度的AS后,观察其对巨噬细胞一氧化氮(NO)合成及对肿瘤细胞杀伤活性的影响;透射电子显微镜观察巨噬细胞的超微结构改变;应用激光扫描共聚焦显微术,结合特异性荧光探针Fluo-3/AM、观察AS引起巨噬细胞内Ca2+的变化。结果:50～800μg/mlAS作用细胞24小时后,NO分泌量增加,杀瘤活性加强,且呈量-效依赖关系;400μg/mlAS作用细胞24小时后,透射电子显微镜观察可见细胞表面突起增多、增粗、增长;滑面内质网、溶酶体增多;50～800μg/mlAS作用细胞4小时后,细胞内Ca2+的浓度升高,并与药物浓度呈正相关。结论:AS能增强小鼠腹腔巨噬细胞的免疫作用;细胞内Ca2+浓度的升高可能是AS激活巨噬细胞,发挥免疫调节作用的机理之一。
Objective： To investigate the immunomodulatory effect of Astragalus Saponin on macrophages and explore the mechanisms of its immunomodulation.Methods：By adding different concentrations of AS into cultured mice peritoneal macrophages, the influence of AS on synthesis of nitro oxide（NO） was observed by NO Kit （enzymic method）.MTr assay was used to determine the cytotoxicity of macrophages induced by AS. The morphological changes of the macrophages were identified by Transmission Electron Microscope. LSCM and specificity fluorescent probe Fluo-3/AM were applied to observe the change of Ca^2＋ in the macrophages induced by AS.Results： AS could significantly increase NO synthesis, enhance the capacity of mice peritoneal macrophages for cytotoxicity to carcinoma ceils. The surface projections of the maerophages were exhibited multiplicating, thickening and extenting via Transmission Electron Microscope. Augmented intracellular Ca^2＋ in the macrophage was observed by LSCM. Conclusion： AS can enhance the immune fimctions of macrophages, the increase of intracellular Ca^2＋ be one of the mechanisms of its immunomodulatory effects.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2008年第9期804-807,共4页
Chinese Journal of Immunology
