摘要
目的:建立以高效液相色谱法同时测定枳实黄酮有效部位中柚皮苷、橙皮苷和新橙皮苷含量的方法。方法:色谱柱为Hy-persil C18(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸水溶液(20:80),流速为1.0mL.min-1,柱温为30℃,检测波长为283nm。结果:柚皮苷、橙皮苷和新橙皮苷的进样量分别在0.44～2.20μg(r=0.9995)、0.0256～0.1280μg(r=0.9993)、0.54～2.70μg(r=0.9999)范围内与各自峰面积积分值呈良好的线性关系;三者平均回收率分别为99.41%、100.33%、99.69%,RSD分别为1.14%、1.47%、1.16%。结论:本方法简便、准确、可靠,可用于枳实的质量控制。
OBJECTIVE： To establish an HPLC method for simultaneous determination of Naringin, Hesperidin and Neohesperidin in the effective fraction of flavone in Fructus Aurantii Immaturus. METHODS： Hypersil C18 column（250 mm × 4.6 mm, 5μm） was used with the mobile phase consisted of acetonitrile -0.1% phosphoric acid solution （20 ： 80） at a flow rate of 1.0 mL · min^- 1, and the detection wavelength was set at 283 nm and the column temperature was maintained at 30 ℃. RESULTS： The calibration curves of Naringin, Hesperidin and Neohesperidin were in good linearity over the ranges of 0.44- 2.20μg（ r = 0. 999 5）, 0. 025 6- 0.128 0 μg （ r = 0. 999 3）, 0.54 - 2.70μg（ r = 0. 999 9）, respectively.And the average recoveries for the three constituents were 99.41%, 100.33% and 99.69%, respectively with RSD at 1.14%, 1.47%, and 1.16%, respectively. CONCLUSION： The method is simple, accurate and reliable, and can be used for the quality control of Fructus Aurantii Immaturus.
出处
《中国药房》
CAS
CSCD
北大核心
2008年第27期2127-2128,共2页
China Pharmacy
关键词
枳实
黄酮
高效液相色谱法
含量测定
Fructus Aurantii Immaturus
Flavanones
HPLC
Content determination
