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高效液相色谱法测定阴炎净洗液中苦参碱的含量 被引量:7

Determination of matrine content in Yinyanjing lotion by HPLC
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摘要 目的建立测定阴炎净洗液中苦参碱含量的方法。方法采用高效液相色谱法,色谱柱VP-ODS(5μm,150mm×4.6mm),流动相为甲醇水(含0.4%三乙胺,磷酸调pH至6.9)(10:90),流速:1mL·min^-1,检测波长:220nm,柱温:42℃。结果苦参碱在线性范围为0.04~0.24mg·mL^-1,回归系数为0.9998,RSD为0.93%,测得苦参碱的平均回收率为99.95%,RSD为0.53%(n=9)。结论本方法简便、准确、可靠,适用于阴炎净洗液中苦参碱的常规分析和质量控制。 Objective To establish an HPLC method for the determination of matrine in Yinyanjing lotion. Methods The column of VP-ODS (5 μm, 150 mm× 4.6 mm) was used. The mobile phase was methanol-water (10: 90, containing 0.4% triethylamine solution, pH adjusted to 6.9 with phosphoric acid). The flow rate was 1 mL · min^-1, the detection wavelength was 220 nm; and the column temperature was 42 ℃. Results There was good linear relationship for matrine at 0. 04-0.24 mg · mL^- 1, r=0. 999 8. RSD=0.93%. The average recovery was 99.94%, RSD=0.53% (n=9). Conclusion The method is simple, accurate, and reproducible. It can be used in the regular analysis and the quality control of matrine in Yinyanjing lotion.
出处 《中南药学》 CAS 2008年第5期559-561,共3页 Central South Pharmacy
基金 2007年湖南省卫生厅科研基金资助课题
关键词 高效液相色谱法 阴炎净洗液 苦参碱 含量测定 HPLC Yinyanjing lotion matrine determination
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