摘要
为了寻找治理水华的有效方法,研究了对羟基苯甲酸对铜绿微囊藻生长的影响以及对鲤鱼的毒性作用.结果表明,一定浓度的对羟基苯甲酸能有效抑制铜绿微囊藻的生长,其半抑制浓度(EC50)为0.42mmol·L-1;抑藻机制之一可能是对羟基苯甲酸能促进细胞氧自由基(O2·-)的产生,从而导致藻细胞膜结构的损伤.实验中抑藻作用最佳浓度的对羟基苯甲酸(0.8mmol·L-1)作用于鲤鱼后,对鲤鱼血清中的天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、γ-谷氨酰基转移酶(γ-GT)、碱性磷酸酶(ALP)的活性没有明显影响,对肝脏、鳃和肌肉中的超氧化物歧化酶(SOD)的活性以及膜脂质过氧化产物(MDA)的含量也没有明显影响,提示对羟基苯甲酸在该浓度下对鲤鱼无毒性作用.因此,对羟基苯甲酸可望被开发出一种高效低毒的生物杀藻剂.
In order to develop new methods to control water bloom,the allelopathic effects of p-hydroxybenzoic acid on Microcystis aeruginosa Kueitz,as well as its acute toxicity toward Cyprinus carpio Linnaeus,were studied.The results showed that the growth of Microcystis aeruginosa Kueitz could be inhibited effectively by p-hydroxybenzoic acid,with an EC50 value of 0.42 mmol · L^-1.The inhibitory effects might be due to the production of oxygen free radicals that led to damage of the algal cell membrane structure.At the concentration with the strongest antialgal effect in this experiment,p-hydroxybenzoic acid showed no acute toxic effects to Cyprinus carpio Linnaeus.There were no significant differences in activities of serum AST,ALT,γ-GT and ALP,as well as liver,gill and muscle SOD activity and MDA content between the treatment group and the control.The results showed that p-hydroxybenzoic acid has potential to be developed into an algicide.
出处
《环境科学学报》
CAS
CSCD
北大核心
2008年第9期1887-1893,共7页
Acta Scientiae Circumstantiae
基金
安徽省教育厅自然科学基金项目(No.2006KJ219B)
生物环境与生态安全安徽省高校重点实验室资助项目~~
关键词
对羟基苯甲酸
化感作用
机制
毒理
酶活性
p-hydroxybenzoic acid
allelopathy
mechanism
toxicity
enzyme activities
