摘要
为寻找生物组织低温无损保存的新方法,研究了磁场对KMnO4溶液冰晶形成的影响.建立了不同形式的磁场发生装置,包括旋转磁场、方波叠加正弦波磁场、50 kHz磁场.为保证样品降温过程慢速均匀,运用虚拟仪器技术建立了温度测控系统.使用低温显微系统对磁场影响下样品冰晶的形态进行了记录.结果表明,低频旋转磁场对生物溶液冰晶的形成产生了影响,尤其50 kHz交变磁场对溶液中冰晶的生长具有较为显著的抑制作用,说明在易于产生的低冷冻速率下,交变磁场对生物组织的低温无损保存研究具有重要意义,适合实际应用.
In order to find new method of non-clToinjury cryopreservation, the influence of magnetic field on ice crystal formation proceess in KMnO4 solution was studied. Different kinds of magnetic generators were applied, such as rotary magnetic field, megnetic field of square wave superposed sine wave, and 50 kHz magnetic field. In order to achieve a slow and even cooling process, a temperature measuring and controling system was developed by applying virtual instrument technology. The shape of ice crystal influenced by magnetic field was recorded by cryo-microscopc instrument. It is showed that low frequency rotary magnetic field affects the ice crystal formation. Especially, 50 kHz alternated magnetic field can significantly suppress the ice crystal growth in KMnO4 solution. This result is very important in the research of non-cryoinjury cryopreservation of organism influenced by special magnetic field in enviroment with slow temperature dropping rate which is easily obtained, and the result is suitable for real application.
出处
《江苏大学学报（自然科学版）》
EI
CAS
北大核心
2008年第5期428-431,共4页
Journal of Jiangsu University：Natural Science Edition
基金
国家自然科学基金资助项目(50307005)
关键词
虚拟仪器
磁场
相变
低温损伤
低温保存
冰晶
virtual instrument
magnetic field
phase transition
cryoinjury
cryopreservation
ice crystal
