摘要
[目的]利用nrDNA ITS序列，探讨18份宁夏枸杞资源的遗传多样性。[方法]采用改进CTAB法提取枸杞叶片基因组DNA，利用合成的特异引物对其DNA中nrDNA ITS区进行扩增、克隆，对目的片段测序分析并对测序结果进行聚类分析。[结果]通过测序首次获得了18种宁夏枸杞nrDNA ITS区碱基序列，整个ITS序列长度变异范围为559～634bp，平均为612bp，整个转录间隔区（ITS1＋ITS2）对位排列后总长度为480bp，包括194个变异住点，占40．4％；286个保守位点，占59．6％。聚类结果表明了18份宁夏枸杞的亲缘关系与差异，并将其分为3个大类。[结论]基于nrDNA ITS区序列分析在研究枸杞种质遗传多样性方面具有一定的优越性。
[ Objective] The study aimed to investigate the genetic polymorphism of 18 Lycium barbarum resources via urDNA ITS sequencing. [ Method] The genomic DNAs from Lycium barbarum leaves were isolated by modified CTAB method for PCR amplification on the nrDNA ITS region using specifically synthesized primers; the amplified fragments were cloned and sequenced,then the sequencing results were clustered. [ Result] nrDNA ITS sequences of the tested 18 Lycium barbarum were firstly obtained in the present study. For whole 18 tested materials,the variation range of whole ITS region is 559-643 bp, with an average of 612 bp; alignment analyses showed that the whole length of internal transcribed spacer （ITS1 ＋ ITS2） was 480 bp, within which there are 194 variation sites,accounting for 40.4% ,and 286 conserved sites,accounting for 59.6%. The cluster results showed that the 18 tested materials could be grouped into three classes. [ Conclusion] Analysis of nrDNA ITS sequence may avail to identify the Lycium barbarum germplasm resources.
出处
《安徽农业科学》
CAS
北大核心
2008年第24期10379-10380,共2页
Journal of Anhui Agricultural Sciences
基金
宁夏自然科学基金资助项目(NZ0769)
关键词
枸杞属
ITS序列
遗传多样性
Lycium Linn.
ITS sequence
DNA sequencing
Genetic polymorphism
