摘要
[目的]为了提高萨福克羊精液低温液态保存的效果。[方法]用葡萄糖、柠檬酸钠、乳糖、卵黄为主要成分配制了5种萨福克羊精液稀释液,分别4、81、21、6倍稀释精液并5℃保存,通过观测精子存活时间、有效存活时间、生存指数等指标,筛选出精液保存时适宜的稀释液、渗透压、pH值及稀释倍数。[结果]4倍稀释不利于精液保存,8、121、6倍稀释精液保存效果好(P<0.01),结合母羊受胎率稀释保存倍数以8倍为宜;以葡萄糖3.0 g、柠檬酸钠2.0 g、卵黄10 ml为主要成分的稀释液保存效果优于其他4种稀释液(P<0.05)。稀释液pH值和渗透压与精液越接近保存效果越好。[结论]调整稀释液成分、稀释倍数、pH值、渗透压,可以提高萨福克羊精液低温液态保存效果。
[ Objective] The researeh aimed to improve the effect of low temperature liquid storage of Suffolk sheep semen. [ Method] Optimal extenders were selected to dilution and chilled storage of Suffolk ram semen through observation of general survival time, available survival time and survival index of sperm. [Result] 4 times dilution was not suited for semen storage, while 8, 12, and 16 times dilution were better than 4 times （ P 〈0.01）. In view of ewe＇s conception, 8 times dilution was better in storage semen. The extender based on glucose 3.0 g, citrate sodium 2.0 g and yolk 20 ml was better for chilled storage than other extenders （ P 〈0.05）. The closer pH value and osmotic pressure of extenders to semen, the better storage effect gained. [ Conclusion] Refining the composition of diluent, dilution multiple, pH value and osmelarity of diluent could improve the effect of low temperature liquid storage of Suffolk sheep semen.
出处
《安徽农业科学》
CAS
北大核心
2008年第25期10887-10889,共3页
Journal of Anhui Agricultural Sciences
基金
新疆兵团科技攻关项目
关键词
萨福克羊
精液
低温保存
Suffolk sheep
Semen
Chilled storage
