摘要
目的研究吸烟、硝酸甘油对动脉粥样硬化血管平滑肌细胞胞内游离钙浓度([Ca2+]i)的影响,探讨吸烟对动脉粥样硬化形成及硝酸甘油的生物效应的作用。方法复制家兔动脉粥样硬化模型,分离血管平滑肌细胞。Fluo-3/AM负载细胞,应用流式细胞仪检测血管平滑肌细胞[Ca2+]i,激光扫描共聚焦显微系统测定单个血管平滑肌细胞内Ca2+的时空变化。结果各组动物主动脉壁均见到程度不同的动脉粥样硬化斑块形成。动脉粥样硬化家兔血管平滑肌细胞[Ca2+]i明显升高(48.45±5.31,与生理盐水对照组38.09±2.57比较,P<0.01);吸烟能增加动脉粥样硬化家兔血管平滑肌细胞[Ca2+]i(56.48±2.99,与单纯动脉粥样硬化组48.45±5.31比较,P<0.01);硝酸甘油则能明显降低动脉粥样硬化家兔血管平滑肌细胞[Ca2+]i(41.91±3.16,与单纯动脉粥样硬化组48.45±5.31比较,P<0.05);吸烟能明显抑制硝酸甘油降低血管平滑肌细胞[Ca2+]i的作用(47.99±5.10,与硝酸甘油组41.91±3.16比较,P<0.05)。结论吸烟能明显增加动脉粥样硬化血管平滑肌细胞[Ca2+]i,硝酸甘油则能明显降低动脉粥样硬化血管平滑肌细胞[Ca2+]i,吸烟能抑制硝酸甘油的生物学效应。
Aim To study the effects of smoking and glonoine on intracellular free Ca^2＋ concentration （[ Ca^2＋ ]i） in vascular smooth muscle cell in rabbits with atherosclerosis, and to explore the effects of smoking on atherosclerosis and biologic action of glonoine. Methods An atherosclerosis in rabbits was produced. The vascular smooth muscle cells were isolated. The cells were loaded by Fluo-3/AM. [ Ca^2＋]i in vascular smooth muscle cell was measured by flow cytometer （FCM）. The spatial distribution and the dynamic changes of [ Ca^2＋]i in single vascular smooth muscle ceils were determined by laser scanning confocal microscopy（LSCM）. Results Atherosclerosis plaques in arteriae aorta were observed and the degrees were dif- ferent in various groups. [Ca^2＋]i in vascular smooth muscle cells in rabbits with atherosclerosis markedly increased[ （48.45 ± 5.31 ） vs that in saline control （38.09 ± 2. 57 ）, P 〈 0. 01 ]. Smoking evidently in- duced the elevation of [ Ca^2＋ ]i in vascular smooth muscle cells in rabbits with atherosclerosis [ （56. 48 ± 2. 99） vs atherosclerosis group （ 48.45 ± 5.31 ）, P 〈 0. 01 ]. Glonoine markedly inhibited the elevation of [ Ca^2＋ ]i in vascular smooth muscle cells in rabbits with atherosclerosis[ （41.91 ± 3.16） vs atherosclerosis group （48.45 ± 5.31 ）, P 〈 0.05 ]. Smoking remarkably suppressed the effect of glonoine on the decrease of [Ca^2＋]i[ （47.99 ± 5.10） vs glonoine group（41.91 ± 3. 16）, P 〈 0. 05 ]. Conclusions Smoking could markedly increase [Ca^2＋]i in vascular smooth muscle cells in rabbits with atherosclerosis. Glonoine markedly inhibited the elevation of [ Ca^2＋]i in vascular smooth muscle ceils in rabbits with atherosclerosis. Smoking remarkably suppressed the biological effect of glonoine.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2008年第9期1224-1228,共5页
Chinese Pharmacological Bulletin
基金
山东省教育厅资助项目(No03K142)
