摘要
为制备对琥珀酸氯霉素分子具有特异性吸附的分子印迹聚合物膜,利用模板分子琥珀酸氯霉素(HS-CAP)、功能单体甲基丙烯酸(MA)、交联剂乙二醇二甲基丙烯酸酯(EGDMA)、引发剂偶氮二异丁腈(AIBN)、溶剂四氢呋喃,采用紫外光引发聚合的方法制备出含有HS-CAP分子印迹位点的印迹聚合微粒,并使用相转化的方法,制备含有这种HS-CAP分子印迹微粒的醋酸纤维素膜.然后通过吸附实验检测该印迹膜的吸附特性,与非印迹膜相比,印迹膜对模板分子具有良好的特异性识别作用,与印迹膜相互作用的模板分子溶液,在作用前后浓度发生了显著的变化;印迹膜对模板分子的识别作用主要集中于与模板分子相互作用的最初2h之内,并随作用时间的延长而降低;当模板分子浓度介于0.2～0.0125mg/mL这一范围内时,模板分子溶液浓度越高,印迹膜的吸附特性越明显.本实验所制备的分子印迹聚合膜对模板分子具有特异性识别能力,可以在下一步研制以分子印迹聚合膜为基础的检测氯霉素残留的传感设备中得到应用.
To prepare the molecularly imprinted membrane with capacity of specifically absorbing the tem- plate chloramphenicol succinate, photo-polymerization method was applied to prepare the molecularly imprinted polymers by using chloramphenicol succinate as template, MA as functional monomer, EGDMA as cross-linking agent, AIBN as initiator and tetrahydrofuran as solvent. Then through phase inversion, cellulose acetate membrane with imprinted sites was made using chloramphenicol succinate imprinted polymers. The absorptive characteristics of this membrane was identified by absorbing experiment. Compared with non-imprinted membrane, imprinted membrane has a good capacity of specifically absorbing the template molecules. The concentration of chloramphenicol succinate solvent changed significantly after being interacted with imprinted membrane. Most of this recognizing reactions were completed in the first 2 h and declined as the reaction time prolonged. The higher the concentration of template in the scope of 0.2-0.0125 mg/mL, the more significant the capacity of the imprinted polymers. The imprinted membrane made in this research had high efficiency towards the separation of template molecule and was proposed to use in the future study for the creation of chloramphenicol detection sensor.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2008年第17期1961-1966,共6页
Acta Chimica Sinica
基金
国家自然科学基金(No.30571368)
国家"863"计划(No.2006AA10Z439)资助项目.
关键词
氯霉素
分子印迹聚合膜
吸附特性
chloramphenicol
molecular imprinted membrane
absorptive characteristics
