摘要
目的评价基因芯片在检测淋球菌(NG)、沙眼衣原体(CT)和解脲脲原体(UU)中的应用价值。方法697例NG检测样本、663例CT检测样本及653例UU检测样本均用现有临床常规检测(培养法检测NG及UU,免疫层析法检测CT)、PCR检测及基因芯片检测。以临床常规检测为参照,分析基因芯片检测与临床常规检测结果的符合情况;以临床常规检测和PCR检测为参照,分析基因芯片检测的敏感性及特异性。结果基因芯片检测结果与临床常规方法的符合率为:NG阳性符合率为99.2%,阴性符合率为97.9%;CT阳性符合率为100%,阴性符合率为97.2%;UU阳性符合率为84.5%,阴性符合率为97.1%。基因芯片检测NG的敏感性为99.1%,特异性为99.3%;基因芯片检测CT的敏感性为98.9%,特异性为99.5%;基因芯片检测UU的敏感性为98.5%,特异性为99.5%。结论基因芯片检测泌尿生殖道NG、UU及CT的特异性和敏感性均较高,在泌尿生殖道病原学诊断中有很好的临床应用价值。
Objective To evaluate the clinical value of a gene chip in the detection of Neisseria gonorrhoeae（NG）, Chlamydia trachomatis（CT） and Ureaplasma urealyticum（UU）. Methods 697 NG clinical detection specimens, 663 CT clinical detection specimens and 653 UU clinical detection specimens were detected by clinical routine tests （ NG and UU with culture method, CT with immunochromatography assay） , PCR assay and the gene chip. Using clinical routine tests as the reference standard, We analyzed the coincident rate between the results detected by the geue chip and clinical routine tests. According to the consist with clinical routine tests and PCR assay, the true positive and true negative samples were identified. Using the gene chip detection results with the true positive and true negative samples, the sensitivity and specificity of the gene chip detection were tested. Results The coincident positive rate and the coincident negative rate of NG were 99.2% and 97.9%, respectively. The coincident positive rate and the coincident negative rate of CT were 100% and 97.2% respectively. The coincident positive rate and the coincident negative rate of UU were 84.5% and 97.1% respective- ly. The sensitivity and specificity of the gene chip for NG were 99.1% and 99.3% respectively. The sensitivity and specificity of the gene chip for CT were 98.9% and 99.5% respectively. The sensitivity and specificity of the gene chip for UU were 98.5% and 99.5% respectively. Conclusion The sensitivity and specificity of the gene chip for NG,UU and CT diagnosis were high. The gene chip is an valuable clinical assay for detection of pathogens of urogenital tract infection.
出处
《皮肤病与性病》
2008年第3期3-6,共4页
Dermatology and Venereology
基金
云南省科技厅中小企业创新基金项目(2006CX14)资助
关键词
基因芯片
淋球菌
沙眼衣原体
解腺支原体
检测
Gene chip
Neisseria gonorrhoeae
Chlamydia trachomatis
Ureaplasma urealyticum
