摘要
目的研究姜黄素对人肝细胞癌耐药株HepG2/ADM耐药逆转作用,并初步探讨其逆转机制。方法通过培养液阿霉素浓度梯度递增法建立HepG2/ADM细胞株,采用MTT法检测其耐药性,免疫组化法检测HepG2/ADM与亲本株P-gp的表达。采用姜黄素作用于HepG2/ADM细胞,MTT法测定其逆转倍数,高效液相色谱法(HPLC)测定细胞内ADM含量。结果HepG2/ADM细胞表现出多药耐药性,P-gp的表达较亲本细胞明显增多,加用姜黄素后HepG2/ADM细胞耐药性有明显逆转,细胞内ADM浓度明显升高。结论姜黄素可以逆转HepG2/ADM细胞的耐药性,提高该细胞对化疗的敏感度,其逆转机制可能与降低P-gp对药物的外排功能,增加细胞内药物浓度有关。
[ Objective ] To evaluate reversal effects of curcumin on HepG2/ADM, multidrug-resistant human hepatoeareinoma cell line, and explore its reversal mechanism. [Methods] HepG2/ADM ceil line was established through increasing ADM with concentration gradient method. The cell viability was determined by MTF. Immunohis- tochemistry was used to detect expression of HepG2/ADM and parents. The effects of eureumin at different concentration on HepG2/ADM cells were observed by microscope and detected by MTT. High performance liquid chro- matography （HPLC） was used to detect concentration of ADM in cells. [Results] HepG2/ADM cell line was estab- lished, which muhi-drug resistance is related to over-expression of P-gp. Increased ceil apoptosis and decreased cell viability followed by treatment of cureumin, which resulted from aceumulation of ADM in HepG2/ADM ceils. [Conclusion] Cureumin can reverse muhidrug resistance of HepG2/ADM cells through inhibiting P-gp-mediated drug efflux and subsequently increased accumulation of drugs.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2008年第17期2441-2443,2448,共4页
China Journal of Modern Medicine
