摘要
采用棒状链霉菌种内原生质体融合技术,选育克拉维酸高产菌株.该实验选用舒巴坦钠耐受性高产菌株Streptomyces clavuligerus B71-3-10和甘油耐受性高产菌株Streptomyces clavuligerus B71-14为亲株,优化了原生质体制备条件和融合条件,最终得到遗传稳定性良好的融合子F14.该融合子的克拉维酸产量提高到650.35 mg/L,分别比亲株S.clavuligerus B71-3-10和S.clavuligerus B17-14的克拉维酸产量高36.77%和20.84%.将原生质体融合技术应用到棒状链霉菌克拉维酸高产菌株的选育中,证明了其可行性、高效性及成效显著性.
Breeding the clavulanic acid high-production strain ofStreptomyces clavuligerus by using interspecific protoplast fusion technique was studied. In this study, sulbactam sodium resistant high-production mutant S.clavuligerus B71-3-10 and glycerin resistant high-production mutant S.clavuligerus B71-14 obtained by the lab in the prophase research were chosen as the parental strains. After the optimization of the protoplast formation and fusion conditions, a genetically stable fusant F14 with higher clavulanic acid production of 650.35 mg/L was obtained finally. The clavulanic acid production of the fusant was 36.77% and 20.84% higher than the parental strain S.clavuligerus B71-3-10 and S.clavuligerus B71-14, respectively. The results shows that using protoplast fusion technique in the breeding of clavulanic acid high-production strain of Streptomyces clavuligerus is feasible, efficient and prominent.
出处
《天津科技大学学报》
CAS
2008年第3期21-25,共5页
Journal of Tianjin University of Science & Technology
基金
天津市自然科学基金资助项目(043802711)
关键词
棒状链霉菌
克拉维酸高产菌株
原生质体融合
Streptomyces clavuligerus
clavulanic acid high-production strain
protoplast fusion
