摘要
从东北红豆杉Taxus cuspidata的主干和侧枝树皮及皮下分离筛选得到181株纯化的内生菌株。经过摇瓶发酵培养,再用甲醇、乙酸乙酯萃取抽提,抽提产物经以标准紫杉醇为对照的薄层层析(TLC)、高效液相色谱(HPLC)、质谱(MS)分析,发现有1株菌株HD181-23能够产生紫杉醇,其紫杉醇产量为206.34μg/L。对菌株HD181-23菌落、菌丝体和孢子形态和产孢特征进行了研究,表明菌株HD181-23为葡萄孢属的种,暂定为Botrytissp.。该菌株的发酵提取纯化产物经HeLa细胞毒实验,结果显示对HeLa细胞具有较强的抑制作用。
One hundred and eighty one purified endophytic fungal strains were isolated and screened from inner bark （phloem-cambium） and xylem of Taxus cuspidata. These strains were fermented and then the fermented solution was extracted using methanol and ethyl acetate. The extracts were analyzed by thin-layer chromatography （TLC）, high performance liquid chromatography （HPLC） and mass spectrum （MS）, using standard taxol as control. The results showed that strain HD181-23 can produce taxol with output of 206.34μg/L. Morphological observation indicates that HD181-23 belongs to the genus Botrytis, and the fungus is tentatively identified as Botrytis sp. The purified products of the fermented extracts have significant inhibitive effects on HeLa cell.
出处
《菌物学报》
CAS
CSCD
北大核心
2008年第5期735-744,共10页
Mycosystema
基金
国家自然科学基金项目(No.30570025)
黑龙江省"十五"重大攻关项目(No.GA02C101)
哈尔滨市青年科学基金项目(No.2005AFQXJ063)
黑龙江省教育厅海外学人科研资助项目(No.1152HZ06)
黑龙江大学杰出青年基金项目
关键词
内生真菌
葡萄孢
红豆杉
发酵提取物
endophytic fungus, Botrytis sp., Taxus cuspidata, fermented extract
