摘要
选用楸树茎段为外植体进行组培腋芽增殖途径研究,结果表明:(1)利于楸树生长的最适宜基本培养基为N6培养基;(2)最佳灭菌方法:取带腋芽茎段,剪成1～2cm,自来水冲洗2h→切剥成0.5cm大小的生长点→超净台上70%酒精30 S→无菌水冲洗4次→0.1%升汞浸6min→无菌水冲洗4次一接种;(3)初代培养最适培养基:N6+6-BA1.0mg/L+NAA0.01mg/L;继代培养最适培养基:N6+6-BA2.0mg/L+NAA 0.1mg/L+Vc100mg/L+稀土2mg/L;生根培养最适培养基:N6+NAA1.0mg/L。
The stem segments with axillary buds of Catalpa bungei were chosen as explants to study its rapid propagation techniques in this paper. The results were as follows ： （ 1 ） The best basic medium was N6 ; （2） The effective procedure was： The one-year-old stem segments were chose and cut into 1 -2 cm sections with one auxiliary, bud each, then washing in tap water for 2h → cutting into 0. 5 cm growing points →soaking in 70% alcohol soaking for 30s → washing in sterile water for 4 times →soaking in 0. 1% HgC12 solution for 6 min → washing in sterile water for 4 times again→ culture in medium; （3） The most suitable medium for primary culture was： N6＋6-BA 1.0 mg/L＋NAA 0.01 mg/L; The most suit- able medium for sub culture was： N6＋6-BA 2.0mg/L＋NAA0. 1 mg/L＋Vc 100 mg/L＋Lanthanide 2 mg/L; The most suitable medium for rooting induction was N6＋NAA1.0 mg/L.
出处
《林业科技开发》
2008年第5期65-68,共4页
China Forestry Science and Technology
基金
"十一五"国家科技支撑计划课题"楸树珍贵用材林培育关键研究与示范"(编号:2006BAD24B08)
江苏省高技术项目"楸树优良无性系选育及苗木标准化生产技术研究"(编号:BG2006319)
关键词
楸树
腋芽
组织培养
快速繁殖
Catalpa bungei
Axillary buds
Tissue culture
Rapid propagation
