摘要
以垂枝早樱‘红枝垂'的带芽茎段为外殖体,接种到MS+1.0mg/L BA+0.1mg/L NAA培养基上,芽的诱导率最高,为80%。采用正交设计方法,研究了BA、NAA、蔗糖3种不同因素及其组合对‘红枝垂'芽增殖系数的影响,结果表明:细胞分裂素BA是幼芽增殖的最主要因素。芽增殖的最佳组合为A_2B_3C_2即MS+0.5mg/L BA+0.1mg/L NAA+35g/L蔗糖,其增殖系数为4.15。当株高在2～3 cm时,转入1/2MS+1.0mg/L NAA+1.0mg/L IBA生根培养基上,接种25 d后,生根率可达95%;经过5～7 d的开瓶炼苗,以沙:珍珠岩:蛭石=1:1:1为移栽基质,20 d后调查,其移栽成活率达84%。
The stem sections with bud of Cerasus subhirtella var. pendula ‘Rosea＇ were selected as explants and were inoculated medium of MS l. 0 mg/L Ns -benzyladenine （BA） and 0. 1 mg/L α-naphthaleneacetic acid （ NAA）, the highest shoot induction rate was 80%. The orthogonal experiment design was used to study the effects of BA, NAA and sucrose on shoot proliferation coefficient of the C. subhirteUa var. pendula ‘Rosea’. The results indicated that BA was the main factor influencing the shoot proliferation. The optimal medium for reproduction of the bud was A2B3C2 treatment, namely, MS with 0. 5 mg/L BA and 0. 1 mg/L NAA ＋ 35 g/L sucrose, and the proliferation coefficient was 4. 15. The rooting rate could reach 95% when the explants of 2 - 3 cm were transplanted in the 1/2 MS medium with 1.0 mg/L indole-3-btyricacid （IBA） and 1.0 mg/L NAA for 25 days. After hardening seedling for 5 - 7 d, the survival rate of planflets reached 84% in the transplant substrate of sand, perlite and vermiculite （ 1 ： 1 ： 1 ） for 20 days.
出处
《林业科技开发》
2008年第5期72-75,共4页
China Forestry Science and Technology
基金
江苏省高校自然科学基金"早樱种系分类学研究"(编号:06KJB180045)
关键词
'红枝垂’
快繁
组织培养
正交试验
Cerasus subhirtella var. pendula ‘ Rosea’
Rapid propagation
Tissue culture
Orthogonal Design
