摘要
背景:研究表明人重组粒细胞集落刺激因子可动员内皮前体细胞,增加脑缺血区域新生血管生成。目的:观察人重组粒细胞集落刺激因子对大鼠脑出血周围区新生血管的影响。设计、时间及地点:随机分组设计的动物对照实验,于2006-03/11在泸州医学院中心实验室完成。材料:健康雄性SD大鼠72只,人重组粒细胞集落刺激因子。方法:72只大鼠按随机数字表法分为3组,假手术组、脑出血组、治疗组,每组24只。断尾取自体血通过鼠脑立体定向仪注入鼠脑内制备脑出血模型,假手术组用生理盐水代替自体血。治疗组于制模后1h腹腔注射人重组粒细胞集落刺激因子60μg/kg。假手术组、脑出血组不做任何处理。主要观察指标:于6,12,24,48,72h,7d6个时间点检测血肿周围区CD34+血管的表达,每个时间点检测4只。利用内皮细胞的标志性抗原CD34变化了解微血管的生成情况,CD34抗原也越多,新生血管越多。结果:脑出血组CD34+血管数明显高于假手术组(P<0.05);治疗组CD34+血管数明显高于脑出血组(P<0.05),且在72h增多明显,与7d比较差异无显著性意义(P>0.05),但与6,12,24,48h比较差异有显著性意义(P<0.05)。结论:人重组粒细胞集落刺激因子能够促进脑出血后血肿周围新生血管生成。
BACKGROUND： Recombinant human granulocyte colony-stimulating factor （rhG-CSF） can mobilize endothelial progenitor cells and enhance new vessels at cerebral ischemia region. OBJECTIVE： To investigate the effects of rhG-CSF on the new microvascular expressions in rat perihematoma after intracerebral hemorrhage. DESIGN, TIME AND SETTING： A randomized control animal experiment was performed at the Central Laboratory of Luzhou Medical College from March to November 2006. MATERIALS： A total of 72 healthy male Sprague Dawley rats and rhG-CSF were used for this study. METHODS： Seventy-two rats were equally and randomly assigned into the sham operation group, the hemorrhage group, the treatment group. According to rat brain stereotaxic atlas, models of intracerebral hemorrhage were made by infusing autoblood from rat tails. Rats in the sham operation group were infused with saline instead of autoblood. Rats in the treatment group were administered rhG-CSF （60 μ g/kg） by intraperitoneal injection at I hour after operation. Rats in the sham operation and hemorrhage groups were left intact. MAIN OUTCOME MEASURES： The microvascular expressions of CD34^＋ in perihematoma were detected at 6, 12, 24, 48 and 72 hours, 7 days; Four rats in each time point. Microvascular production was measured by changes in CD34. The more the CD34 antigens, the more the new vessels were. RESULTS： In the hemorrhage group, the microvascular expressions of CD34^＋ were significantly higher compared to the sham operation group （P 〈 0.05）. In the treatment group, the microvascular expressions of CD34^＋ were significantly higher compared to the hemorrhage group （P 〈 0.05）, and the microvascular expressions of CD34^＋ were significantly increased at 72 hours, and no significant difference was detected compared to at 7 days （P 〉 0.05）. Significant differences were measured at 6, 12, 24 and 48 hours （P 〈 0.05）. CONCLUSION： The rhG-CSF may enhance the microvascular expressions in peribematoma after hemorrhage.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第38期7539-7542,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
