摘要
目的比较不同方法提取的柿叶总黄酮含量。方法分别采用水提取法、传统乙醇提取法、微波提取法、超临界CO2萃取法提取柿叶总黄酮,并用紫外分光光度计在510 nm处测定总黄酮的含量。结果水提取法测得总黄酮含量为0.721%,传统乙醇提取法测得总黄酮0.948%,微波辅助提取法测得总黄酮含量0.908%,超临界CO2萃取法测得总黄酮含量0.961%。结论超临界CO2萃取法提取总黄酮含量最高,传统乙醇提取法提取总黄酮含量次之,微波辅助提取法再次之,水提取法提取总黄酮含量最低。
Objective： To compare different extraction methods for persimmon leaf total flavonoids. Methods： The contents of persimmon leaf total flavonoids were determined by using the method of water-extraction, traditional ethanol-extracon and supercritical carbon dioxide-extraction, and UV spectrophotometer was used to detect the total flavonoids in 510 nm. Results： The content in the water-extraction was O. 721%, the content in the traditional ethanolextraction was 0.948%, the content in microwave-extraction was 0. 908% and the content in supercritical carbon dioxideextraction was 0. 961%. Conclusion： The content by the method of supercritical carbon dioxide-extraction is the highest, the second by the traditional ethanol-extraction, the third by the microwave-extraction and the lowest by the water-extraction.
出处
《泰山医学院学报》
CAS
2008年第6期454-455,共2页
Journal of Taishan Medical College
关键词
柿叶总黄酮
提取方法
含量比较
persimmon leaf total flavonoids
extraction method
comparison of content
