摘要
目的：应用ISSR-PCR标记技术研究不同种及居群莪术的亲缘关系和遗传多样性。方法：对蓬莪术、广西莪术和温郁金共8个居群的37个样本进行ISSR-PCR分析，用POPGEN32软件分析其遗传相似系数及遗传距离，UPGMA方法聚类，构建亲缘关系系统图。结果：5条引物共扩增出65个位点，其中34个位点是多态性位点，多态位点百分率为52．3％。遗传相似系数变化范围0．6864～0．9997。Neig基因多样性（H）指数为n1521，Shannon信息指数（I）为0．2338，莪术种内的遗传多样性低于种间。结论：莪术居群间的遗传变异较大，而居群内部的分化程度很低。不同居群的莪术与种的特性和地理分布有一定相关性，为莪术的品种鉴定及栽培育种提供了分子生物学依据。
Objective： To study the genetic diversity and genetic relationship in different species and populations of Curcuma by ISSR-PCR marker technique. Method： Eighty populations and 37 samples of Curcuma including C. phaeocaulis, C. kwangsiensis and C. wenyujin were studied by ISSR-PCR markers. The systematic diagram of Similar coefficient and genetic distance were set up by POPGEN32 software and clustered by UPGMA method. Result： A total of 65 loci were scored by 5 primers, among which 34 were polymorphic loci. The percentage of polymorphic loci was 52.3%. Genetic similarity coefficient changed from 0. 686 4 to 0. 999 7. Nei＇s gene diversity index（H）, and Shannon information index（I） were 0. 152 1 and 0.233 8. The inner genetic diversity of Curcurna species was lower than the outer. Conclusion： The genetic variation of different populations Curcuma was big. The inherited differentiation of inner populations was low. Different populations of Curcuma were related to character of species and geological distribution.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第18期2037-2040,共4页
China Journal of Chinese Materia Medica
基金
浙江省科技攻关重大项目(2005C13019)
教育部科技创新工程重大项目培育资金(706018)
关键词
不同种
居群
莪术
ISSR
different species
populations
Rhizoma Curcumae
ISSR
